一株高分子量多环芳烃降解菌的筛选、鉴定及降解特性研究

Isolation and Characterization of a High-molecular-weight (HMW) PAHs Degrading Bacterial Strain

摘要:

采用富集培养方法从多环芳烃污染土壤中筛选分离得到1株能以苯并[a]芘(B[a]P)为唯一碳源和能源生长的菌株。形态特征观察和16S rDNA序列分析结果表明, 该菌株为副球菌属(Paracoccus sp.), 编号为HPD-2。HPD-2在3.0 mg/L的B[a]P液体培养基中生长较慢, 培养5 d后B[a]P的降解率为89.7%。同时, 该菌株对四环的芘和荧蒽也具有较好的降解能力, 培养7 d后芘和荧蒽的降解率分别达到47.2%和84.5%。可见, 该菌株对高分子量PAHs具有很好的降解潜力。

Abstract:

A bacterial strain, which utilizied Benzo[a]pyrene (B[a]P) as the sole carbon and energy source for growth, was isolated from soil heavily contaminated by PAHs. This strain was identified as Paracoccus sp. by its morphology and 16S rDNA sequence, and designated HPD-2. After incubated in MS medium containing B[a]P of 3.0 mg/L for 5 days, 89.7% of B[a]P was degraded by HPD-2. When this strain was grown in pyrene and fluoranthene of 50 mg/L for 7 days, 47.2％and 84.5% of which was degraded respectively. Thus, this study provides evidence for the potential application of HPD-2 for improving HMW- PAHs biodegradation.