一个甲烷氧化菌株的分离、鉴定及其特性研究

Isolation, Identification and Characterization of a Methanotrophic Strain

摘要:

实验室自行设计方案分离多株以甲烷为唯一碳源的菌株, 对其中的1株QJ16进行了研究, 根据该菌株形态特征与16S rDNA序列的同源性分析, 证实该菌株是一个与其最近的甲基单胞菌属各成员都不相同的菌株。对该菌株的培养条件和利用甲烷的特性进行研究结果表明, 氮源以氯化铵和硝酸钾共同作用最好, 碳源以甲烷最佳, 最佳生长温度为30℃, 最佳生长pH为6~7; 在批式实验时菌株利用甲烷的最适pH为6.5左右, 微量元素Cu2+的浓度为15 mmol/ L。

关键词:甲烷氧化细菌, 甲烷, 16S rDNA测序, 分离鉴定, 特性

Abstract:

Strain QJ16, one of some methanotrophic bacteria isolated from soil samples utilizing the separate device designed by our laboratory; was studied in detail and identified as one of the Genus methylomonas through 16S rDNA sequencing and comparison. The culturing and reaction conditions for methane utilization of this strain were investigated. The results indicated that the optimal conditions for the growth of strain QJ16 were methane gas as carbon source and the coexistence of NH4Cl and KNO3 as nitrogen source, the cultivation temperature of 30℃ and medium initial pH 6~7, and the optimal Cu2+ concentration of 15 mmol/L.

Key words:Methanotrophic bacteria, Methane, 16S rDNA sequencing, Isolation and identification, Characterization