Biodegradability evaluation for caster oil-based lube oil was carried out in the test. By repeated experiments, a basic analytical method for degradability of the lube oil was established and compared with the routine experimental method. The results showed that the basic analytical method was more logical and more reliable, and the experimental data have higher repeatability than the previous ones. Under the optimal culture conditions for the targeting strains 5-11-4, and according to the basic method, the degradation rate of the lube oil arrived to 88.37%, approximate 10.25 percent higher than the corresponding yield obtained by the routine experimental method (the value was about 78.12%), indicating the difference between the basic analytical method and the routine experimental one.