科微学术

微生物学通报

产碱性纤维素酶兼性厌氧菌株的筛选和酶学性质的初步研究
作者:
基金项目:

陕西省科学院青年基金项目(No. 2006k-30)


Screening of Facultative Anaerobic Strain Producing Alkaline Cellulase and Study on Enzymatic Properties
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献 [20]
  • |
  • 引证文献
  • | |
  • 文章评论
    摘要:

    用CMC平板筛选方法, 从造纸厂碱性土壤中获得产碱性纤维素酶兼性厌氧菌株LZ-5, 革兰氏阴性, 经鉴定为弧菌属(Vibrio sp.)中的一个种。液体摇瓶培养产生碱性纤维素酶活力高达5.8 U/mL。酶学性质初步研究显示, LZ-5产生的纤维素酶最适反应温度为40℃, 最适反应pH值为9.0, 在碱性条件下具有较高的酶活性和较好的稳定性。经传代培养证实该菌株遗传性能稳定。

    Abstract:

    The Gram-negative, facultative anaerobic strain, LZ-5 producing alkaline cellulase had been screened and isolated from the alkaline soil of paper mill. The strain was identified to be a kind of genus Vibrio. The cellulase activity of culture liquid was 5.8 U/mL.The primary study on enzymatic properties showed: optimum temperature of 40℃, optimum pH of 9.0, with stable enzymatic activity under alkaline condition. The strain LZ-5 was tested by the experiments of subculture and the results showed that the strain LZ-5 had genetic stability.

    参考文献
    引证文献
引用本文

李忠玲,王卫卫,任 平,赵文娟,张红艳,陈卫锋. 产碱性纤维素酶兼性厌氧菌株的筛选和酶学性质的初步研究[J]. 微生物学通报, 2008, 35(6): 0851-0854

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
文章二维码