产碱性纤维素酶兼性厌氧菌株的筛选和酶学性质的初步研究

Screening of Facultative Anaerobic Strain Producing Alkaline Cellulase and Study on Enzymatic Properties

摘要:

用CMC平板筛选方法, 从造纸厂碱性土壤中获得产碱性纤维素酶兼性厌氧菌株LZ-5, 革兰氏阴性, 经鉴定为弧菌属(Vibrio sp.)中的一个种。液体摇瓶培养产生碱性纤维素酶活力高达5.8 U/mL。酶学性质初步研究显示, LZ-5产生的纤维素酶最适反应温度为40℃, 最适反应pH值为9.0, 在碱性条件下具有较高的酶活性和较好的稳定性。经传代培养证实该菌株遗传性能稳定。

关键词:碱性纤维素酶, 兼性厌氧, 革兰氏阴性菌

Abstract:

The Gram-negative, facultative anaerobic strain, LZ-5 producing alkaline cellulase had been screened and isolated from the alkaline soil of paper mill. The strain was identified to be a kind of genus Vibrio. The cellulase activity of culture liquid was 5.8 U/mL.The primary study on enzymatic properties showed: optimum temperature of 40℃, optimum pH of 9.0, with stable enzymatic activity under alkaline condition. The strain LZ-5 was tested by the experiments of subculture and the results showed that the strain LZ-5 had genetic stability.

Key words:Alkaline cellulase, Facultative anaerobic, Gram-negative strain