利用门多萨假单胞菌NK-01合成中长链聚羟基脂肪酸酯(PHAMCL)

Synthesis of Medium-chain-length Polyhydroxyalkanoate (PHAMCL) by Pseudomonas mendocina NK-01

摘要:

从土壤中分离到1株能够合成中长链聚羟基脂肪酸酯(PHAMCL)的细菌, 经分类鉴定命名为Pseudomonas mendocina NK-01。对于该菌株在氮源限制的情况下, 利用葡萄糖合成的PHAMCL进行了结构分析。同时, 获得了该菌株PHA合成酶基因phaC1, 利用大肠杆菌表达载体pBluescript SK—构建了重组载体pBSphaC1, 并在E.coli JM109中成功表达。以所构建的工程菌株进行了发酵合成。

Abstract:

A strain which can synthesize medium-chain-length polyhydroxyalkanoate (PHAMCL) was isolated from soil. It was named as Pseudomonas mendocina NK-01 through classification and identification. The PHAMCL was synthesized by P. mendocina NK-01 from glucose in the condition of limited nitrogen source. The structure of it was characterized. In order to construct a recombined plasmid pBSphaC1, an expressing plasmid of E.coli, after phaC1 gene encoding PHA synthase was cloned from Pseudomonas mendocina NK-01 by PCR amplification, it was linked into pBluescript SK—. PhaC1 gene was expressed successfully in E.coli JM109. Fermentation synthesis was also investigated by recombined E.coli.