连云港台北和盐城三圩盐田土壤嗜盐菌多样性研究

Polymorphic Halophilic Bacteria and Haloarchaea from Lianyungang Taibei and Yancheng Sanwei Salt Field Soils

摘要:

盐田土壤嗜盐微生物对盐田生态系统的良性循环和盐的生产至关重要。本文对江苏连云港台北盐田土壤和盐城三圩盐田土壤的嗜盐细菌和古菌的多样性进行了研究, 结果表明两地盐土嗜盐细菌和古菌的分布具有相似性和独特性。采用培养法从两地盐土中共分离到17株嗜盐细菌, 其中Halomonas为两地盐土共有的嗜盐细菌, 而Halobacillus和Pontibacillus仅在三圩盐土中发现。通过非培养的16S rDNA 基因文库法从两地盐土中发现了13种嗜盐古菌, 台北盐土有Halobacterium 和 Haloplanus, 三圩盐土有Halobacterium, Natronobacterium, Halogeometricum 和 Haloarcula。10个嗜盐古菌的16S rDNA和GenBank已知序列的同源性为92%~97%, 可能为这些属中的新种。该研究为盐田环境嗜盐微生物资源的开发和利用奠定了基础。

Abstract:

Halophilic microorganisms play important rules in salt field ecosystem and salt production. In this study, halophilic bacteria and haloarchaea from soils of Lianyungang Taibei and Yancheng Sanwei salt fields were analyzed. The halophilic bacterial and haloarchaeal types from both the soils were similar, but each soil had its distinctive species. A total of 17 halophilic bacteria were identified, among them, Halomonas was found from both the soils, while Pontibacillus and Halobacillus were isolated from Sanwei salt field only. Using uncultured 16S rRNA gene library technology, 13 haloarchaeal soil 16S rRNA genes were identified from both the saline soils. Halobacterium and Haloplanus were found from Taibei salt field, while Halobacterium, Natronobacterium, Halogeometricum and Haloarcula were identified from Sanwei salt field. Ten haloarchaeal 16S rRNA gene sequences showed 92%~97% identities with the GenBank sequences that appear to represent novel soil haloarchaeal species. This study provides important information that is useful for further investigation and application of halophiles of saline soil fields.