一株DDT降解菌的筛选、鉴定及降解特性的初步研究

Isolation and Characterization of a DDT Degradation Bacterium Strain D-1

摘要:

从DDT污染的土壤中筛选具有DDT降解能力的细菌, 经过富集培养、分离纯化得到56株细菌, 将其接种到基础盐酵母培养基, 7 d后用紫外分光光度计法初筛得到降解率较高的一株菌, 编号为D-1。通过16S rDNA序列分析结合传统分类学方法确定该菌为寡养单胞菌属(Stenotrophomonas sp.)的一株菌。对菌体降解DDT的特性的研究表明, 在培养温度为30℃, 底物质量浓度为40 mg/L, pH 7.0, 摇床转速为200 r/min的条件下, 该菌株对DDT降解10 d的降解率为69.0%。

关键词:寡养单胞菌, DDT降解, 16S rDNA

Abstract:

A bacterium strain D-1 capable of degrading DDT was isolated from the DDT contaminated soil．Based on the phenotype, physiological and biochemical characteristics, and the phylogenetic analysis of 16S rDNA sequence, the strain D-1 was identified preliminarily as Stenotrophomonas sp.. This strain could degrade DDT under condition of 30℃, pH7.0, 200 r/min, with degradation efficiency of 69.0% in 10 days.

Key words:Stenotrophomonas sp., DDT degradation, 16S rDNA