生物滤塔体系好氧反硝化菌的分离鉴定与特性研究

Separating and Studying of the Aerobic Denitrifying Bacteria from Biofilter

摘要:

从实验室生物滤塔填料的生物膜上, 经选择性培养基筛选, 分离出4株好氧反硝化菌。好氧状态下4种菌的40 h反硝化率均大于80%, 其中菌种A1反硝化率可达到99.05%。跟踪菌种反硝化过程中氮元素24 h变化过程, 发现4株菌除A1外都有亚硝酸根积累。菌种A1为短杆菌, 革兰氏阴性。生理生化特性研究与16S rDNA 序列测定(GenBank接受号DQ836052.1)初步判定菌种A1为假单胞菌Pseudomonas putida。适于菌A1生长的初始pH值是7.0左右, 温度30℃左右, 当DO大于2.0 mg/L时, DO的变化对菌种A1的反硝化效果影响很小。

Abstract:

Four strains of aerobic denitrifying bacteria were isolated from biomembrane of the laboratory biofilter by selective medium. The denitrifying rates of these four strains were found more than 80% under aerobic condition for 40 hours. The denitrifying rate of A1 was highest, which was 99.05%. When measuring the course of nitrogen element changing, it is found that they accumulated nitrite expect the strain A1. The strain A1 was gram positive and spherical. It is identified as Pseudomonas putida based on its biochemical and morphological characters and phylogenetic analysis of 16S rDNA sequences(genbank accession NO.DQ836052.1). For the strain A1, the optimum beginning pH was 7.0 around, and the optimum temperature was 30℃ around, The change of DO did not influence the effect of denitrification when it was more than 2.0 mg/L.