采用3种方法对不同酵母菌的总DNA进行提取, 经琼脂糖凝胶电泳、ND-1000 型微量紫外分光光度计检测以及酶切、连接、预扩增试验结果的分析, 表明采用改良氯化苄法提取酵母菌的总 DNA 质量最好, 基本无 DNA 碎带, 蛋白质污染小。通过对野生酵母菌菌株的验证试验, 进一步证明采用改良氯化苄法提取的总 DNA 产量稳定、重复性好, 可以直接用于限制性内切酶酶切试验, 完全适于 AFLP 分析及一些其它分子生物学研究的要求。
Abstract:
The high molecular weight genomic DNA of yeast was extracted using three methods. Products were separated on agarose gel electrophoresis, quantified by spectrophotometer ND-1000 and restricted by EcoRⅠand MesⅠ. The result was shown that the genomic DNA extracted by modified benzyl chloride method was the best. The products of wild isolates supported it, too. This method was suitable for restriction of genomic DNA from yeast.
