The fast development of the molecular biology and the further research on the nucleic acid set a solid foundation for the development of genosensor. Genosensor is the result of combining molecular biology with microelectronics, electrochemistry, optics and etc, which will build a bridge between the life science and the information science and become one of the most important technologies for DNA information analysis and detection. The working principle, classification of genosenor and the recent research on its application in the detection of functional genes of environmental microorganisms are discussed according to the latest literature. And it is pointed out that the application in the determination of microorganism functional genes in compost is an important development orientation of genosensor.