杆状病毒表面展示系统研究进展

The Advancement about Baculovirus Surface Display System

摘要:

杆状病毒表面展示系统是近年发展起来的一种新的真核生物表面展示系统。通过与病毒衣壳或囊膜蛋白融合表达, 可以将外源肽展示在杆状病毒表面, 形成刺猬状“伪病毒”。该文结合作者本人的实际工作, 简要介绍此系统的原理、特点, 并综述其在单抗制备、新型疫苗研制、基因转导与基因治疗等领域的最新研究进展。相信经过改进与优化, 其可展现出更广阔的应用前景。

Abstract:

At present, microbe surface display system mainly involves phage surface display system, bacterial surface display system, yeast surface display system and virus surface display system. Baculovirus surface display system is a new type of eukaryote surface display system which developed based on deeply understanding of construction and function of virus genome in recent years. Through fused expression with viral capsid or membrane proteins exogenous peptides can be displayed on the surface of the virus and formed hedgehog-shape “fake virus”. Baculovirus surface display system was characterized by safeness and high performance, furthermore, this system can complete post-translation processing and modification of protein to enhance the bioactivity of exogenous product. Combined with the author’s experimental work, this paper briefly introduces the mechanism and traits of this system and summarizes the newest research development on its application in the field of monoclonal antibody preparation, new-type vaccine development, genes transduction and genes therapy. It is believed that the system above may show extensive application through further improvement and optimization.