农用抗生素TS99高产菌株的选育

Screening of Agriantibiotic TS99 High-production Strain

摘要:

在明确链霉素对农抗TS99产生菌Streptomyces fungicidicus YH04孢子的致死浓度为1.2 mg/mL的基础上, 以链霉素致死浓度为选择压力, 采用不同剂量的紫外线照射对菌株孢子进行诱变处理, 获得了大量的链霉素抗性基因突变株, 进而从中筛选到发酵效价较出发菌株提高60％以上, 且遗传稳定性良好的高产菌株Streptomyces fungicidicus YH9407。

Abstract:

Streptomycin-resistant mutants were obtained from original strains YH04 treated by UV (253 nm, 30 W, 45 s or 60 s) under the selection pressure of resistance to streptomycin (the lethal concentration is 1.2 mg/mL). Streptomyces Strain YH9407 with high antibiotic yield and genetic stability was obtained from streptomycin-resistant mutants. The yield of antibiotic TS99 is more than 60% higher than that of the original strain.