聚乙烯醇高效降解菌的筛选及降解特性研究

Study on Screening and Degradation Character of Degrading Bacterium for Polyvinyl Alcohol

摘要:

以聚乙烯醇为唯一碳源从环境中筛选获得了高效降解聚乙烯醇的微生物菌株XT11, 初步鉴定为假单胞菌属(Pseudomonas sp.)。对菌株Pseudomonas XT11的生长过程及PVA降解过程进行了研究, 发现该菌株在54 h内可将1 g/L的聚乙烯醇(PVA)降解。同时研究了温度、pH值及酵母膏浓度对该菌株降解PVA的影响, 结果表明其最适温度、pH值和酵母膏浓度分别为30℃、7.0和0.5 g/L。研究了PVA浓度对PVA降解率的影响, 发现随着PVA浓度的增大, PVA的降解率降低。

Abstract:

A strain XT11 was isolated from environment, which could degrade polyvinyl alcohol. It belongs to the genus of Pseudomonas sp. The course curve of growth and PVA degradation were discussed. The results showed that 1 g/L of PVA could be completely degraded after 54 h cultivation. The initial pH of fermentation medium, culture temperature and the concentration of yeast extract were also discussed. The results showed that the optimum initial pH, culture temperature and the concentration of yeast extract were 7.0, 30℃ and 0.5 g/L respectively. Effect of the concentration of PVA on PVA degradation was discussed. The results showed that PVA degradation decreased as the concentration of PVA increased.