Diathesis education and innovative ability cultivating of students are a new position in higher education. Exploring experiment was applied in medical undergraduates of grade 2004 for enhancing integrative diathesis and cultivating innovative spirit and ability of students. It was proved that application of exploring experiment could increase adequately learning fervor and interest of students. Teaching quality of microbiological experiment was enhanced accordingly. Application of exploring experiment was useful supplement and attempt of traditional teaching mode.