喀斯特生境台湾相思根瘤菌的生物学特性研究

Biological Characteristics of the Rhizobia Isolated from Acacia confusa Grew in the Karst Environment

摘要:

从广西喀斯特地区台湾相思树采集根瘤并分离纯化获得15个根瘤菌株, 对其生物学特性进行了研究, 结果表明: 这些根瘤菌均具有典型的根瘤菌的个体形态特征。3-酮基乳糖反应、牛肉膏蛋白胨生长均呈阴性; 淀粉水解、柠檬酸盐利用等均呈阳性; 在BTB反应和石蕊牛奶反应均产酸; 都能利用磷酸二氢铵和所供试的7种糖类; 11个菌株能使硝酸盐还原; 有14个菌株能在3.0%和4.0%浓度的NaCl下生长良好或较好; 39℃下有14个菌株能生长; 有13个菌株在pH12条件下能生长, 4个菌株在pH4的酸性条件下能生长; 15个菌株都能在≤10%的CaCO3浓度下生长, 其中有11个菌株能在10%~30%的钙浓度内生长。表现出在喀斯特环境下, 根瘤菌的生理生化特性及抗逆性的多样性。

Abstract:

The paper is about the systematic studies of biological characteristics of 15 stains rhizobia isolated purified from Acacia confusa grew in Guangxi karst environment. The results showed that there were typical characteristics of rhizobia .there were negative reaction about use of 3-ketolactose and beef extract peptone nutrient agar medium ,and positive reaction about use of starch and citrate medium ,and produce acid in reaction of BTB and litmus milk medium, (NH4 )2HPO4 was used as nitrogen sources and both four monosaccharides and three disaccharides could be utilized as carbon sources in 15 strains rhizobia isolated Acacia confuse .Among the 15 strains for the tests, 11 strains could deoxidize the nitrate of medium into nitrite, 14 strains could grow well on NaCl solution concentration 3.0 % ~ 4.0 % , 14 strains could grow at 39℃, 13 strains may grow on highest pH12 and 4 strains on lowest pH4 cultrue medium. 15 strains can grow in 10% and 11 strains in 10%~30% of CaCO3 solution concentration.