腐蚀生物膜垢中硫酸盐还原菌的系统进化分析

Lineages Evolution Analysis of Sulfate-reducing Bacteria in the Corrosion Biofilm

摘要:

从油田回注水系统加压泵后的管道管壁生物膜垢里分离得到的一株嗜热、嗜酸、异养型硫酸盐还原菌, 编号为CW-04。该菌株兼性厌氧、革兰氏阳性、有极生鞭毛、直或弯曲的短杆状,能产孢子, 最佳生长温度47℃, 在20℃~63℃下培养基中均能使测试瓶有黑色沉淀产生。从腐蚀防治的角度研究该菌株的生长曲线。主要生理生化特性包括: 生长pH范围为3.7~10, 最佳生长pH为6~6.5, 最佳生长盐度为0.7%。能够在H2+醋酸盐、甲酸盐、乙酸盐、丙酸盐、乳酸盐、丙酮酸盐和乙醇等电子供体中生长, 也能够利用葡萄糖、果糖和简单的氨基酸作为唯一碳源生长; 能够在硫酸钠、硫代硫酸钠、亚硫酸钠等电子受体中生长, 但是不能利用硝酸盐和硫磺。通过16S rDNA测序以及系列比对分析: 该菌属于细菌界(Bacteria)、厚壁菌门(Firmicutes)、梭菌纲(Clostridia)、梭菌目(Clostridiales)、蛋白胨链球菌科(Peptococcaceae)、脱硫肠状菌属(Desulfotomaculum), 与该属中的种Desulfotomaculum aeronauticumd 相似性达到99%。

Abstract:

A new thermophilic, acidityphilic, sulfate-reducing heterotrophic bacteria, strain CW-04, was isolated from biofilm dirtiness in pipeline wall after press pump of affusion system in oilfield. The bacterium is an concurrently anaerobic, non-motile, Gram-positive, pole flagellum, spore-forming curved rod growing within a pH range of 3.7~10(optimal growth at pH 6~6.5) and temperature range of 20℃~63℃(optimal growth at 47℃), then, growth curve was studied to efficiently prevent corrosion. The optimum NaCl concentration for growth is 0.7%. A limited number of compounds are utilized as electron donors, including H2+acetate, formate, acetate, lactate, propionate, pyruvate and ethanol. Sulfate, sulfite and thiosulfate, but not sulfer or nitrate, can be used as electron acceptors. Strain CW-04 is able to utilize glucose, fructose and simple aminophenol as sources of carbon. Analysis of the 16S rDNA sequence allowed strain CW-04(accession numbers: AY703033) to be classified as a representative of a new strain of the genus Desulfotomaculum, Desulfotomaculum aeronauticumd (Bacteria, Firmicutes, Clostridia, Clostridiales, Pep-tococcaceae) whose similarity is 99%.