转小鼠金属硫蛋白-Ⅰ基因聚球藻7002的生长潜力分析

Preliminary Studies on the Growth Potential of Transgenic Synechococcus sp. PCC 7002 with Mouse Metallothionein-I Gene

摘要:

以野生聚球藻7002为对照, 从室温吸收光谱、光合放氧速率、生长动力学参数以及气升式光生物反应器中的生长特性阐述了转小鼠金属硫蛋白-Ⅰ基因聚球藻7002的生长优势和培养潜力。结果表明: 转MT聚球藻室温可见光光谱吸收峰比野生藻略高; 最大净光合速率和饱和光强比野生藻高, 呼吸速率和补偿光强比野生藻低; 转MT聚球藻摇瓶培养的最大细胞浓度为野生藻的1.74倍, 具有较高的细胞生长速率; 气升式光生物反应器有利于转MT基因聚球藻生长潜力的发挥。

Abstract:

The growth predominance and culturing potential of transgenic Synechococcus sp. PCC 7002 with mouse metallothionein-Ⅰgene are characterized and expatiated by room temperature absorption spectra, photosynthetic oxygen evolution rate, growth kinetic parameters. The results show that the spectral absorption, maximum net photosynthetic rate and saturated light intensity of transgenic Synechococcus sp. PCC 7002 are higher than wild strain, whereas its the respiration rate and compensating light intensity are lower than wild strain. The transgenic cells exhibit a higher growth rate, and the maximum cell concentration is 1.74 times higher than wild ones when cultured in shaking flask. The air lift photobioreactor is suitable for bringing its growth potential into play.