紫外诱变改良柑橘采后病害拮抗菌柠檬形克勒克酵母研究

Ultraviolet Mutation Breeding Aspect of the Biological Control of Postharvest Diseases by Kloeckera apiculata in Citrus Fruit

摘要:

以柠檬形克勒克酵母(Kloeckera apiculata)为出发菌株, 采用紫外线(UV)、UV+LiCl等方法对其进行诱变改良。确定了处理的最佳剂量：紫外处理15 W 30 cm照射20 s, UV+LiCl处理UV照射20 s并在平板中加入LiCl 0.3% (W/V)。选育到一株生理特性有明显改善的变异菌株UV20-13, 果实试验中, 7 d后柑橘青、绿霉病的发病率分别比出发菌株降低25.56%和10.00%。生长动态测定和传代试验表明, 该菌株生长特性优于出发菌株, 连续传代10代, 该菌株没有出现退化、回复突变等, 在遗传上是稳定的。

关键词:柑橘, 采后病害, 柠檬形克勒克酵母, 诱变育种, 紫外线

Abstract:

A series of experiments were conducted to study the mutation of Kloechera apiculata by many kinds of treatments such as UV and UV+LiCl. The optimal dosage disposal was determined: 15 W 30 cm under Ultraviolet irradiation for 20 s, UV+LiCl under Ultraviolet irradiation for 20 s and added LiCl 0.3% (w/v). One strain (UV20-13) which had obvious physiological characteristic was obtained. the incidence of blue and green mold of citrus was reduced by 25.56% and 10.00% in vivo experiment after 7 days respectively. The strain UV20-13 was tested by the experiments of subculture and dynamic growth, and the results showed that the strain UV20-13 was better than K.apiculata in the growth characteristics, and it did not appear retrogression, reversion mutation ect after subculturing 10 generations. Therefore the strain UV20-13 had genetic stability.

Key words:Citrus, Postharvest diseases, Kloeckera apiculata, Mutation breeding, Ultraviolet radiation