五种益生菌在纤维固定化连续培养系统的共存定殖

Stable Coexistence of Five Bacterial Strains as a Community in an Immobilized Continuous Culture System

摘要:

模拟肠道的半固态生境, 建立以玉米纤维为载体的固定化连续搅拌培养系统、并以非固定化连续搅拌培养系统为对照, 稀释率为0.0417, 分别连续培养12 d, 双歧杆菌、酪酸菌、嗜酸乳杆菌、肠膜芽孢杆菌、粪肠球菌五种菌在两种系统中均可稳定共存, 固定化系统固相五种菌数均高于非固定化系统, 以双歧杆菌增幅最大, 半固态的固定化连续培养较液态连续培养的菌相与肠道菌相较一致, 能更好地模拟肠道微生态。扫描电镜观察五种菌在玉米纤维上固定形成了生物膜。

关键词:固定化, 连续培养, 混合培养, 玉米纤维

Abstract:

Five kinds of probiotic bacteria are immobilized and cultured continuously in simulated intestinal systems with corn fiber as the carrier. The biomass and the colonization in biofilm are investigated by selective culture methods and scanning electron microscope. Bifidobacterium longum TQ21-2-2, Lactobacillus acidophile CICC06005, Clostridum butyric TO-A, Bacillus mesentericus TO-A and Enterococcus faecalis T-110 can coexist stably . The biomass of five probiotics in the immobilized phase is greater than that of liquid in the continuous shake flask reactor. Five kinds of probiotic bacteria formed biofilm on corn fiber which be observed by SEM. Continuous culture of immobilized cells could be used better model than that of liquid , the model can be used to study for microecology and microecologocal agent.

Key words:Immobilization, Continuous culture, Mixed culture, Probiotics