黄绿木霉菌产纤维素酶条件优化

The Optimal Condition of Producing Celluase by Trichoderma aureoviride

摘要:

利用正交设计方法研究了温度、接种量、pH值、装液量等不同发酵条件对黄绿木霉菌产纤维素酶的影响, 研究结果表明, 在这些因素中影响该菌株产纤维素酶的最主要因素为温度, 而其他三个因素对该菌株产纤维素酶影响比较小。研究中得出该菌株最适产酶条件为发酵5d, 28℃、初始pH为6、接种量为8%、装液量为40 mL (150 mL三角瓶), 摇床转数为170 r/min。

Abstract:

Producing cellulase conditions such as different temperature, inoculum size, liquid level and pH level by Trichoderma aureoviride in the shaking bottle were studied by orthogonal design method. The results showed that the main factor affecting the producing cellulase was temperature among the orthogonal conditions. The optimum conditions were as follows: cultivating solution initial pH was 6, cultivating temperature was 28℃, inoculation size was 8%, liquid level was 40 mL in 150 mL triangle bottle, rotation speed was 170 r/min.