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具抗菌活性海洋放线菌菌株JMC 06001的分离和鉴定
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国家重点基础研究发展计划“973项目”(No. 2004CB719601); 福特基金项目(07JDPHE031, 07JDPHE053); 吉首大学人才引进基金项目; 湖南省教育厅科学研究项目(C05168)


Isolation and Identification of Marine ActinomyceteJMC06001 Exhibiting Strong Antibacterial Activity
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    摘要:

    从湛江硇洲岛高盐泥样中分离到一株微嗜盐放线菌JMC 06001, 该菌株的发酵产物具有很强的抗菌活性。菌株JMC 06001在多数培养基上生长良好, 气生菌丝白色, 基内菌丝淡黄色至浅棕色。在燕麦琼脂 (ISP 3)、甘油-天门冬酰胺琼脂 (ISP 5)、葡萄糖-天门冬酰胺琼脂及马铃薯浸汁琼脂上产生黄色可溶性色素, 在酵母膏-麦芽膏琼脂 (ISP 2)、蛋白胨酵母膏铁盐琼脂 (ISP 6)和营养琼脂上产生棕色可溶性色素。生长温度范围为4℃~40℃, 最适生长温度28℃。生长pH 范围为6.0~9.0, 最适pH 7.0。能在含0~1.5 mol/L NaCl的培养基上生长, 最适生长盐浓度为0.2~0.5 mol/L NaCl。根据其形态学特征、生理生化特征、细胞化学分类特征和基于16S rRNA基因序列的系统发育分析结果, 菌株JMC 06001被鉴定为链霉菌属(Streptomyces)的有效发表种波赛链霉菌(S. peucetius)的一个菌株。

    Abstract:

    One slightly halophilic marine actinomycete strain JMC 06001 was isolated from a saline mud sample collected from the Island Naozhou in the South China Sea, near Zhanjiang, a city of southern China. The fermentation broth of strain JMC 06001 strongly inhibited the growth of Staphylococcus aureaus, Sarcina lutea, Bacillus subtilis, Escherichia coli, Micrococcus luteus and Proteus vulgaris. The combination of morphology, physiological and biochemical characteristics, chemotaxonomic data and 16S rRNA gene sequence analysis supported the view that strain JMC 06001 belong to a known species of the genus Steptomyces, S. peucetius. The strain studied grown well on most media tested, with white aeriel hyphae and pale yellow to pale brown substance hyphae. Yellow diffusible pigments were produced on oatmeal agar (ISP 3), potato extract agar and glucose/asparagines agar, and pale brown to deep brown diffusible pigments were produced on yeast extract/malt extract agar (ISP 2), glycerol/asparagine agar (ISP5), peptone-yeast ext-Fe agar (ISP 6) and nutrient agar. Growth occurred at 4 ℃~40 ℃ and pH 6.0~9.0, with optimum growth at 28 ℃ and pH 7.0. The tolerant range of NaCl was 0~1.5 mol/L, with best growth occurring in media containing 0.2~0.5 mol/L NaCl.

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陈义光,张晓蓉,张 丽,刘祝祥,彭德姣,肖怀东,黄 苛,陈奇辉,徐丽华. 具抗菌活性海洋放线菌菌株JMC 06001的分离和鉴定[J]. 微生物学通报, 2008, 35(1): 40-44

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