葡萄果粒表皮酵母菌多样性研究

Species Diversity of Yeasts on the Surface of Grape

摘要:

从新疆、甘肃、陕西、宁夏、山东主要酿酒葡萄产区收集葡萄果粒并分离得到酵母258株, 利用26S rDNA的D1/D2区序列分析并结合形态学、生理学特征对这些菌株进行了分类学研究, 探讨了这些地区葡萄果粒表皮酵母的物种多样性及其分布。共鉴定出13属26种, 其中优势属为汉逊酵母属Hanseniaspora(5种), 假丝酵母属Candida(4种), 毕赤酵母属Pichia(4种)和伊萨酵母属Issatchenkia(2种)。对分离自不同地域的同种内不同菌株进行了D1/D2序列分析比较, 以探讨不同地理起源地酵母种内序列稳定性及其变异。

关键词:酵母菌, 多样性, 26S rDNA D1/D2, 序列分析

Abstract:

258 yeast strains were isolated from the surface of grapes,collected from wine grape cultivation areas in Xinjiang, Gansu, Shaanxi, Ningxia and Shandong Provinces.These strains were identified mainly based on the 26S rDNA D1/D2 domain sequence analysis and morphological and physiological characterization. Among them, 26 species belonging to 13 genera were recognized. The dominant genera identified were Hanseniaspora (5 species), Candida (4 species), Pichia (4 species) and Issatchenkia(2 species). D1/D2 sequence variation were compared among the strains of the same species from different areas.

Key words:grape yeast, species diversity, 26S rDNA D1/D2 domain, sequence analysis