Abstract:[Background] Soil harbors abundant filamentous fungi which are of great significance for the research on biodiversity and the development of new bioactive compounds. [Objective] To investigate the soil fungal diversity in some areas of China and reserve biological resources. [Methods] Fungal strains were isolated by the dilution-plate method from soil samples. The colony characteristics and microscopic morphology of these strains were observed, measured, described and graphed. The genomic DNA was extracted from mycelia, and the ITS and LSU regions were sequenced. Bayesian inference and maximum likelihood methods were employed to construct phylogenetic trees. [Results] Based on the morphological characteristics and molecular data, three Hypocreales species, Neoacremonium minutisporum, Parasarocladium wereldwijsianum and Sarocladium sasijaorum were identified. [Conclusion] Referring to the reported Hypocreales species in China, we confirmed these three species as new records in China. The results enrich the species diversity of fungi in China.
