科微学术

微生物学通报

白酒酿造环境中高温菌株的筛选、鉴定及其特性分析
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北京市自然科学基金(6222003)


Screening, identification, and characterization of thermophilic strains in the brewing environment of Baijiu
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    摘要:

    【背景】高温菌株在白酒酿造环境中普遍存在,它们是白酒酿造工艺中不可或缺的一类关键微生物菌群。【目的】对白酒酿造环境样品中的高温菌株进行分离、纯化及鉴定,并深入研究它们的生长特性,为其在白酒生产中的应用提供科学依据和理论基础。【方法】对多份酒曲、酒醅和酒糟样品分别进行梯度稀释,然后选取合适稀释梯度样品分别涂布在溶菌肉汤琼脂培养基、酵母浸出粉胨葡萄糖琼脂培养基和马铃薯葡萄糖琼脂培养基上,通过三区划线分离方法进行筛选。【结果】共筛选获得22株高温细菌和18株高温真菌;通过菌落形态观察分析发现有4种细菌和4种真菌菌落形态不同,分别选取这8种微生物进行鉴定,结合形态观察、生理生化特性和分子生物学鉴定为白色链霉菌(Streptomyces albus)、枯草芽孢杆菌(Bacillus subtilis)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、鲁戈斯芽孢杆菌(Bacillus rugosus) 4种高温细菌和微小根毛霉(Rhizomucor pusillus)、嗜热棉毛菌(Thermomyces lanuginosus)、坚脆嗜热子囊菌(Thermoascus crustaceus)、樟绒枝霉(Malbranchea cinnamomea) 4种高温真菌,其中鲁戈斯芽孢杆菌和樟绒枝霉在白酒研究中尚无报道;对上述8种不同高温微生物菌种生长温度范围、pH范围和NaCl耐受性等分析发现,不同菌种生物学特性存在差异。【结论】本研究共筛选到4种不同的高温细菌和4种不同的高温真菌,并对其进行鉴定以及生长特性分析,为后续解析这些微生物在白酒酿造中的作用及应用提供了重要保障。

    Abstract:

    [Background] Thermophilic strains are ubiquitous in the brewing environment of Baijiu and are a microbial group indispensable for the brewing of Baijiu. [Objective] To isolate and identify thermophilic strains in the samples collected from the brewing environment of Baijiu and characterize the growth of the strains, thereby laying a theoretical foundation for the application of the strains in the production of Baijiu. [Methods] Gradient dilution was carried out for the samples of Jiuqu (starter), Jiupei (fermented grains), and Jiuzao (distilled grains), respectively. The samples with suitable dilution factors were then selected and coated on the Luria-Bertani, yeast extract peptone dextrose, and potato dextrose agar media, respectively. The plate streaking method was employed to isolate the strains. [Results] A total of 22 strains of thermophilic bacteria and 18 strains of thermophilic fungi were screened out. Among them, 4 species of bacteria and 4 species of fungi with distinct colony morphology were selected and identified by morphological observation, physiological and biochemical tests, and phylogenetic analysis. The four species of bacteria were identified as Streptomyces albulus, Bacillus subtilis, B. amyloliquefaciens, and B. rugosus, and the four species of fungi were identified as Rhizomucor pusillus, Thermomyces lanuginosus, Thermoascus crustaceus, and Malbranchea cinnamomea. It is worth noting that B. rugosus and M. cinnamomea had not been reported in the research on Baijiu. The eight species showcased differences in the growth temperature range, pH range, and NaCl tolerance. [Conclusion] We screened out, identified, and characterized the growth of four species of thermophilic bacteria and four species of thermophilic fungi, which provided data support for studying the roles and applications of these microorganisms in the brewing of Baijiu.

    参考文献
    [1] 牛姣, 沈毅, 张贵虎, 程伟, 李贺贺, 郑福平, 孙宝国. 白酒酿造原料与酒体品质关系的研究进展[J]. 食品与发酵工业, 2023, 49(3): 322-328. NIU J, SHEN Y, ZHANG GH, CHENG W, LI HH, ZHENG FP, SUN BG. Research progress on the relationship between raw materials and Baijiu quality[J]. Food and Fermentation Industries, 2023, 49(3): 322-328(in Chinese).
    [2] 马冲, 刘淼, 李涵, 蔡小波, 何旭, 黄孟阳, 秦辉. 现代微生物技术在白酒酿造中的应用研究进展[J]. 酿酒科技, 2023(5): 117-122, 127. MA C, LIU M, LI H, CAI XB, HE X, HUANG MY, QIN H. Research progress in the application of modern microbial technology in Baijiu production[J]. Liquor-Making Science & Technology, 2023(5): 117-122, 127(in Chinese).
    [3] ZHANG HX, WANG L, WANG HY, YANG F, CHEN LQ, HAO F, LV XB, DU H, XU Y. Effects of initial temperature on microbial community succession rate and volatile flavors during Baijiu fermentation process[J]. Food Research International, 2021, 141: 109887.
    [4] ZHENG XW, TABRIZI MR, ROBERT NOUT MJ, HAN BZ. Daqu: a traditional Chinese liquor fermentation starter[J]. Journal of the Institute of Brewing, 2011, 117(1): 82-90.
    [5] SHANG CH, LI YJ, ZHANG J, GAN SL. Analysis of bacterial diversity in different types of Daqu and fermented grains from Danquan distillery[J]. Frontiers in Microbiology, 2022, 13: 883122.
    [6] FAN GS, FU ZL, TENG C, WU QH, LIU PX, YANG R, MINHAZUL KAHM, LI XT. Comprehensive analysis of different grades of roasted-sesame-like flavored Daqu[J]. International Journal of Food Properties, 2019, 22(1): 1205-1222.
    [7] 汪江波, 沈玉洁, 张明春, 曹敬华, 陈茂彬. 白云边高温大曲中嗜热功能菌的分离筛选[J]. 酿酒, 2010, 37(5): 28-31. WANG JB, SHEN YJ, ZHANG MC, CAO JH, CHEN MB. Isolation and differentiation of thermophilic Bacteria in Baiyunbian high temperature Daqu[J]. Liquor Making, 2010, 37(5): 28-31(in Chinese).
    [8] FAN GS, FU ZL, SUN BG, ZHANG YH, WANG XL, XIA YQ, HUANG MQ, LI XT. Roles of aging in the production of light-flavored Daqu[J]. Journal of Bioscience and Bioengineering, 2019, 127(3): 309-317.
    [9] WANG Q, WANG CY, XIANG XQ, XU HL, HAN GQ. Analysis of microbial diversity and succession during Xiaoqu Baijiu fermentation using high-throughput sequencing technology[J]. Engineering in Life Sciences, 2022, 22(7): 495-504.
    [10] CHEN YR, LI KM, LIU T, LI RY, FU GM, WAN Y, ZHENG FP. Analysis of difference in microbial community and physicochemical indices between surface and central parts of Chinese special-flavor Baijiu Daqu[J]. Frontiers in Microbiology, 2021, 11: 592421.
    [11] ZHA MS, SUN BG, YIN S, MEHMOOD A, CHENG L, WANG CT. Generation of 2-furfurylthiol by carbon-sulfur lyase from the Baijiu yeast Saccharomyces cerevisiae G20[J]. Journal of Agricultural and Food Chemistry, 2018, 66(9): 2114-2120.
    [12] CHAI LJ, LU ZM, ZHANG XJ, MA J, XU PX, QIAN W, XIAO C, WANG ST, SHEN CH, SHI JS, XU ZH. Zooming in on butyrate-producing clostridial consortia in the fermented grains of Baijiu via gene sequence-guided microbial isolation[J]. Frontiers in Microbiology, 2019, 10: 1397.
    [13] ZHA MS, YIN S, SUN BG, WANG X, WANG CT. STR3 and CYS3 contribute to 2-furfurylthiol biosynthesis in Chinese sesame-flavored Baijiu yeast[J]. Journal of Agricultural and Food Chemistry, 2017, 65(27): 5503-5511.
    [14] WANG YP, WEI XQ, GUO XW, XIAO DG. Effect of the deletion of genes related to amino acid metabolism on the production of higher alcohols by Saccharomyces cerevisiae[J]. BioMed Research International, 2020, 2020(1): 6802512.
    [15] YANG L, ZENG SR, ZHOU MD, LI YT, JIANG ZY, CHENG PY, ZHANG CL. Comprehensive genomic and metabolomic analysis revealed the physiological characteristics and pickle like odor compounds metabolic pathways of Bacillus amyloliquefaciens ZZ7 isolated from fermented grains of Maotai-flavor baijiu[J]. Frontiers in Microbiology, 2023, 14: 1295393.
    [16] SUN H, CHAI LJ, FANG GY, LU ZM, ZHANG XJ, WANG ST, SHEN CH, SHI JS, XU ZH. Metabolite-based mutualistic interaction between two novel clostridial species from pit mud enhances butyrate and caproate production[J]. Applied and Environmental Microbiology, 2022, 88(13): e0048422.
    [17] ZHANG HZ, ZHANG LJ, YU XW, XU Y. The biosynthesis mechanism involving 2, 3-pentanedione and aminoacetone describes the production of 2-ethyl-3, 5-dimethylpyrazine and 2-ethyl-3,6-dimethylpyrazine by Bacillus subtilis[J]. Journal of Agricultural and Food Chemistry, 2020, 68(11): 3558-3567.
    [18] ZHANG YJ, SUN Q, LIU XY, BASIT RA, MA JH, FU ZL, CHENG LJ, FAN GS, TENG C. Screening, identification, and fermentation condition optimization of a high-yield 3-methylthiopropanol yeast and its aroma-producing characteristics[J]. Foods, 2024, 13(3): 418.
    [19] MA JH, CHENG LJ, ZHANG YJ, LIU YC, SUN Q, ZHANG J, LIU XY, FAN GS. Screening of yeasts isolated from Baijiu environments for producing 3-methylthio-1-propanol and optimizing production conditions[J]. Foods, 2022, 11(22): 3616.
    [20] FAN GS, LIU PX, CHANG X, YIN H, CHENG LJ, TENG C, GONG Y, LI XT. Isolation and identification of a high-yield ethyl caproate-producing yeast from Daqu and optimization of its fermentation[J]. Frontiers in Microbiology, 2021, 12: 663744.
    [21] BUCHANAN RE, GIBBONS NE. 伯杰细菌鉴定手册[M]. 中国科学院微生物研究所, 译. 8版. 北京: 科学出版社, 1984 BUCHANAN RE, GIBBONS NE. Bergey’s Manual of Determinative Bacteriology[M]. Institute of Microbiology, Chinese Academy of Sciences, trans. 8th ed. Beijing: Science Press, 1984(in Chinese).
    [22] 蔡妙英, 东秀珠. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001. CAI MY, DONG XZ. Handbook for Identification of Common Bacterial Systems[M]. Beijing: Science Press, 2001(in Chinese).
    [23] BARAKI SG, SIAHKOLAEE SN, MOUSAVI A. Optimization of the genomic DNA extraction in some mosses[J]. Rostaniha, 2018, 19(2): 165-175.
    [24] 秦立芹, 马景浩, 李二浩, 周阳, 杨博思, 朱宇婷, 吴秋华, 田进英, 李秀婷, 范光森. 一株源于北派酱香白酒酿造环境中产淀粉酶细菌的筛选、鉴定及其特性研究[J]. 中国酿造, 2021, 40(11): 154-160. QIN LQ, MA JH, LI EH, ZHOU Y, YANG BS, ZHU YT, WU QH, TIAN JY, LI XT, FAN GS. Isolation and identification of an amylase-producing bacterium from Beipai sauce-flavor Baijiu brewing environment and its characteristics research[J]. China Brewing, 2021, 40(11): 154-160(in Chinese).
    [25] 朱会芳, 江正强, 赵兰英, 郝姗姗. 一株产木聚糖酶的耐冷皮壳正青霉菌鉴定与酶谱分析[J]. 应用与环境生物学报, 2009, 15(3): 376-379. ZHU HF, JIANG ZQ, ZHAO LY, HAO SS. Identification of a novel cold-adaptive xylanolytic Eupenicillium crustaceum and zymogram analysis of xylanase[J]. Chinese Journal of Applied & Environmental Biology, 2009, 15(3): 376-379(in Chinese).
    [26] 范光森, 许岱, 富志磊, 许春艳, 滕超, 孙宝国, 李秀婷. 古井贡酒曲中酵母的分离及其产香挥发性风味物质分析[J]. 中国食品学报, 2018, 18(7): 220-229. FAN GS, XU D, FU ZL, XU CY, TENG C, SUN BG, LI XT. Screen of aroma-producing yeast strains from Gujinggong Daqu and analysis of volatile flavor compounds produced by them[J]. Journal of Chinese Institute of Food Science and Technology, 2018, 18(7): 220-229(in Chinese).
    [27] 范光森, 严烨, 杨绍青, 闫巧娟, 江正强. 一株产木聚糖酶嗜热真菌樟绒枝霉的鉴定及其产纤维质降解酶系分析[J]. 应用与环境生物学报, 2013, 19(1): 48-53. FAN GS, YAN Y, YANG SQ, YAN QJ, JIANG ZQ. Identification of a xylanase-producing thermophilic fungus, Malbranchea cinnamomea, and analysis of its cellulosic enzymes[J]. Chinese Journal of Applied and Environmental Biology, 2013, 19(1): 48-53(in Chinese).
    [28] 蔡杰华, 刘斌, 石冬梅, 彭万强, 赖钧灼. 四种嗜热真菌的分离与鉴定[J]. 现代生物医学进展, 2012, 12(6): 1059-1064. CAI JH, LIU B, SHI DM, PENG WQ, LAI JZ. Isolation and identification of four species of thermorphilic fungi[J]. Progress in Modern Biomedicine, 2012, 12(6): 1059-1064(in Chinese).
    [29] 杨瑞娟, 吕杰, 严亮, 杨柳霞, 李晨晨, 姜姝, 盛军. 普洱茶渥堆发酵中嗜热真菌的分离和鉴定[J]. 茶叶科学, 2011, 31(4): 371-378. YANG RJ, LU J, YAN L, YANG LX, LI CC, JIANG S, SHENG J. Isolation and identification of thermophilic fungi during the fermentation of Puer tea[J]. Journal of Tea Science, 2011, 31(4): 371-378(in Chinese).
    [30] 周森, 胡佳音, 崔洋, 赵卫鹏, 张如今, 白逢彦. 应用高通量测序技术解析清香型大曲微生物多样性[J]. 中国食品学报, 2019, 19(6): 244-250. ZHOU S, HU JY, CUI Y, ZHAO WP, ZHANG RJ, BAI FY. Microbial diversity analysis of flight-flavor Daqu using high-throughput sequencing[J]. Journal of Chinese Institute of Food Science and Technology, 2019, 19(6): 244-250(in Chinese).
    [31] 张溪桐, 徐岩, 杜海. 低含水量条件下大曲中抑制土味素的功能微生物解析[J]. 食品与发酵工业, 2022, 48(13): 55-62. ZHANG XT, XU Y, DU H. Analysis of the geosmin-inhibiting microorganisms in Daqu under low water content[J]. Food and Fermentation Industries, 2022, 48(13): 55-62(in Chinese).
    [32] 尤小龙. 酱香白酒酿造过程放线菌代谢活性成分研究[D]. 贵阳: 贵州大学硕士学位论文, 2016. YOU XL. Study on active components of a actinomycete from Maotai-flavor liquor brewing process[D]. Guiyang: Master’s Thesis of Guizhou University, 2016(in Chinese).
    [33] 姚亚林, 郑若欣, 程铁辕, 邓杰, 任志强, 卫春会, 黄治国. 浓香型白酒窖泥中放线菌的发酵特性研究[J]. 食品研究与开发, 2020, 41(13): 191-197. YAO YL, ZHENG RX, CHENG TY, DENG J, REN ZQ, WEI CH, HUANG ZG. Study on the fermentation characteristics of actinomycetes in pit mud of Luzhou-flavor liquor[J]. Food Research and Development, 2020, 41(13): 191-197(in Chinese).
    [34] 白成松, 陈莉, 卢红梅, 周莲, 任勰柯. 茅台地区酱香型酒糟中高温放线菌的分离鉴定[J]. 食品工业科技, 2017, 38(8): 199-202. BAI CS, CHEN L, LU HM, ZHOU L, REN XK. Isolation and identification of thermoactinomycete from Moutai-flavor vinasse in Moutai region[J]. Science and Technology of Food Industry, 2017, 38(8): 199-202(in Chinese).
    [35] 张靖宜, 张倩茹, 项文化, 魏树和, 牟文燕. 一株拮抗立枯丝核菌的放线菌筛选、鉴定及生理特性[J]. 生态学杂志, 2014, 33(2): 394-399. ZHANG JY, ZHANG QR, XIANG WH, WEI SH, MU WY. Screening, identification and physiological characteristics of an actinomycete strain against Rhizoctonia solani[J]. Chinese Journal of Ec of Yunnan University, 2012(in Chinese).
    [36] 林群, 肖之陶, 付秋香, 方向明, 莫龙久, 潘启福. 产香风味地衣芽孢杆菌(Bacillus licheniformis)的分离及发酵代谢产物分析[J]. 酿酒科技, 2013(12): 49-52. LIN Q, XIAO ZT, FU QX, FANG XM, MO LJ, PAN QF. Isolation of aroma-producing Bacillus licheniformis and analysis of its fermentation metabolites[J]. Liquor-Making Science & Technology, 2013(12): 49-52(in Chinese).
    [37] 张璋, 赵腾飞, 李红霞, 宋露露. 产蛋白酶芽孢杆菌的筛选、耐酸性驯化及蛋白酶酶学性质分析[J]. 中国酿造, 2024, 43(1): 84-90. ZHANG Z, ZHAO TF, LI HX, SONG LL. Screening and acid resistance acclimatization of protease- producing Bacillus and enzymatic characteristics analysis[J]. China Brewing, 2024, 43(1): 84-90(in Chinese).
    [38] 成冬冬, 董灿灿, 韩小龙, 迟玉洁, 吕冰, 杨威, 陈坤, 胡彦营. 浓香型大曲白酒窖池微生物及养护研究进展[J]. 食品科技, 2022, 47(12): 15-19. CHENG DD, DONG CC, HAN XL, CHI YJ, LYU B, YANG W, CHEN K, HU YY. Research progress on microorganism and maintenance of Luzhou-flavor Daqu liquor pit[J]. Food Science and Technology, 2022, 47(12): 15-19(in Chinese).
    [39] SHEN T, LIU J, WU Q, XU Y. Increasing 2-furfurylthiol content in Chinese sesame-flavored Baijiu via inoculating the producer of precursor l-cysteine in Baijiu fermentation[J]. Food Research International, 2020, 138(Pt A): 109757.
    [40] HE GQ, DONG Y, HUANG J, WANG XJ, ZHANG SY, WU CD, JIN Y, ZHOU RQ. Alteration of microbial community for improving flavor character of Daqu by inoculation with Bacillus velezensis and Bacillus subtilis[J]. LWT, 2019, 111: 1-8.
    [41] BHATTACHARYA D, de LOS SANTOS VILLALOBOS S, RUIZ VV, SELVIN J, MUKHERJEE J. Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L.[J]. Antonie van Leeuwenhoek, 2020, 113(11): 1675-1687.
    [42] 孔晓双, 黄新新, 董应宏, 侯敏, 买尔哈巴·艾合买提, 侯新强, 崔卫东. 一株产β-葡萄糖苷酶甘草内生菌的筛选、全基因组分析及产酶优化[J]. 微生物学通报, 2024, 51(1): 279-294. KONG XS, HUANG XX, DONG YH, HOU M, MARHABA A, HOU XQ, CUI WD. Screening, whole genome analysis, and fermentation condition optimization of a β-glucosidase-producing endophyte from Glycyrrhiza uralensis Fisch[J]. Microbiology China, 2024, 51(1): 279-294(in Chinese).
    [43] 高颖, 张弘弢, 陈媛媛. 一株枯草芽孢杆菌的分离鉴定及其体外降解AFB1的效果分析[J]. 黑龙江八一农垦大学学报, 2022, 34(5): 54-60. GAO Y, ZHANG HT, CHEN YY. Isolation and identification of a Bacillus subtilis FWJ16X degrading aflatoxin B1 and detoxification effect in vitro[J]. Journal of Heilongjiang Bayi Agricultural University, 2022, 34(5): 54-60(in Chinese).
    [44] 张颖超, 于鑫, 赵祥颖, 刘丽萍, 黄艳红, 张家祥, 刘建军. 产4-乙基愈创木酚菌株的筛选、鉴定及其在酱油酿造中的应用[J]. 中国酿造, 2023, 42(11): 115-121. ZHANG YC, YU X, ZHAO XY, LIU LP, HUANG YH, ZHANG JX, LIU JJ. Screening and identification of 4-ethyl guaiacol-producing strain and its application in soy sauce brewing[J]. China Brewing, 2023, 42(11): 115-121(in Chinese).
    [45] 江森, 李仁祥, 李博, 唐霄, 李童, 薛文阁, 尹崇. 艾草发酵物中凝结芽孢杆菌的分离鉴定及生物学特性[J]. 湖南饲料, 2023(6): 38-43. JIANG S, LI RX, LI B, TANG X, LI T, XUE WG, YIN C. Isolation, identification and biological characteristics of Bacillus coagulans from wormwood fermentation products[J]. Hunan Feed, 2023(6): 38-43(in Chinese).
    [46] 卢宇, 马东晓, 周锦涛, 胡治旭, 杨泽慧, 段晓盟, 纵丹, 何承忠. 一株产吲哚乙酸的日本野漆树内生枯草芽孢杆菌生长条件及其促生特性研究[J]. 云南农业大学学报(自然科学), 2023, 38(3): 392-399. LU Y, MA DX, ZHOU JT, HU ZX, YANG ZH, DUAN XM, ZONG D, HE CZ. Growth conditions and growth-promoting characteristics of an endophytic bacterium from Toxicodendron succedaneum[J]. Journal of Yunnan Agricultural University (Natural Science), 2023, 38(3): 392-399(in Chinese).
    [47] 胡江涛, 刘奇, 周英, 宋倩, 何小露, 郭崇炎, 邹志华, 梅文宇, 熊春晖, 张静燕. 芽孢杆菌hjt6的鉴定、生长特性及其对瓠瓜灰霉病菌的拮抗效果[J]. 微生物学通报, 2024, 51(3): 832-845. HU JT, LIU Q, ZHOU Y, SONG Q, HE XL, GUO CY, ZOU ZH, MEI WY, XIONG CH, ZHANG JY. Identification and growth characterization of Bacillus sp. hjt6 with antagonistic effects on Cladosporium tenuissimum causing gray mold disease of bottle gourd[J]. Microbiology China, 2024, 51(3): 832-845(in Chinese).
    [48] HONG LX, FU GM, LIU T, CHEN YR, WU SW, CAI WQ, XIE ZJ, WAN Y. Functional microbial agents enhance ethanol contents and regulate the volatile compounds in Chinese Baijiu[J]. Food Bioscience, 2021, 44: 101411.
    [49] 吴正坤, 刘蒲临, 杨团元, 缪礼鸿, 廖卫芳. 不同贮存期高温大曲微生物群落演替与理化指标相关性分析[J]. 中国酿造, 2023, 42(7): 160-166. WU ZK, LIU PL, YANG TY, MIAO LH, LIAO WF. Correlation analysis of microbial community succession and physicochemical properties of Daqu in different storage periods[J]. China Brewing, 2023, 42(7): 160-166(in Chinese).
    [50] STERGIOU PY, FOUKIS A, GKINI OA, BAROUNI E, GEORGOULIA PS, KANELLAKI M, KOUTINAS AA, PAPAGIANNI M, PAPAMICHAEL EM. Novel FRET-substrates of Rhizomucor pusillus rennin: Activity and mechanistic studies[J]. Food Chemistry, 2018, 245: 926-933.
    [51] 范光森, 杨绍青, 闫巧娟, 严烨, 江正强. 嗜热棉毛菌固体发酵产木聚糖酶条件的优化[J]. 食品工业科技, 2012, 33(6): 219-224. FAN GS, YANG SQ, YAN QJ, YAN Y, JIANG ZQ. Optimization of xylanase production from Thermomyces lanuginosus CAU44 by solid-state fermentation[J]. Science and Technology of Food Industry, 2012, 33(6): 219-224(in Chinese).
    [52] HUANG YH, YI ZL, JIN YL, ZHAO YG, HE KZ, LIU DY, ZHAO D, HE H, LUO HB, ZHANG WX, FANG Y, ZHAO H. New microbial resource: microbial diversity, function and dynamics in Chinese liquor starter[J]. Scientific Reports, 2017, 7: 14577.
    [53] LI J, ZHANG JD, SHEN SG, ZHANG B, YU WW. Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis[J]. Materials Today Communications, 2020, 24: 101197.
    [54] ZHU Q, CHEN LQ, PENG Z, ZHANG QL, HUANG WQ, YANG F, DU GC, ZHANG J, WANG L. The differences in carbohydrate utilization ability between six rounds of Sauce-flavor Daqu[J]. Food Research International, 2023, 163: 112184.
    [55] 黄娜, 张明, 李子健, 饶家权, 邹永芳, 黄丹, 罗惠波. 不同颜色酱香型大曲微生物群落结构及功能比较[J]. 食品与发酵工业, 2024, 50(4): 260-266. HUANG N, ZHANG M, LI ZJ, RAO JQ, ZOU YF, HUANG D, LUO HB. Comparative analysis of microbial community structure and function in Jiangxiang Daqu with different colors[J]. Food and Fermentation Industries, 2024, 50(4): 260-266(in Chinese).
    [56] GARBIN AP, GARCIA NFL, CAVALHEIRO GF, SILVESTRE MA, RODRIGUES A, Da PAZ MF, FONSECA GG, LEITE RSR. β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential[J]. Anais da Academia Brasileira de Ciências, 2021, 93(1): e20191349.
    [57] LIU X, GUO KL, ZHANG HX. Determination of microbial diversity in Daqu, a fermentation starter culture of Maotai liquor, using nested PCR-denaturing gradient gel electrophoresis[J]. World Journal of Microbiology and Biotechnology, 2012, 28(6): 2375-2381.
    [58] FAN GS, YANG SQ, YAN QJ, GUO Y, LI YX, JIANG ZQ. Characterization of a highly thermostable glycoside hydrolase family 10 xylanase from Malbranchea cinnamomea[J]. International Journal of Biological Macromolecules, 2014, 70: 482-489.
    [59] HAN P, ZHOU P, HU SQ, YANG SQ, YAN QJ, JIANG ZQ. A novel multifunctional α-amylase from the thermophilic fungus Malbranchea cinnamomea: biochemical characterization and three-dimensional structure[J]. Applied Biochemistry and Biotechnology, 2013, 170(2): 420-435.
    [60] BASOTRA N, JOSHI S, SATYANARAYANA T, PATI PK, TSANG A, CHADHA BS. Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics[J]. International Journal of Biological Macromolecules, 2018, 108: 185-192.
    [61] ZHU N, JIN HM, KONG XP, ZHU YY, YE XM, XI YL, DU J, LI BQ, LOU MH, SHAH GM. Improving the fermentable sugar yields of wheat straw by high-temperature pre-hydrolysis with thermophilic enzymes of Malbranchea cinnamomea[J]. Microbial Cell Factories, 2020, 19(1): 149.
    [62] 姚灿, 李国友, 张彬, 郝传发, 杨涛. 中高温大曲中嗜热真菌的分离鉴定及其酶活性测定[J]. 酿酒, 2019, 46(5): 32-35. YAO C, LI GY, ZHANG B, HAO CF, YANG T. Isolation and identification of thermophilic fungi from moderate/high-temperature Daqu and determination of enzyme activity[J]. Liquor Making, 2019, 46(5): 32-35(in Chinese).
    [63] 杨越. 云南部分温泉嗜热微生物的多样性及嗜热酶的研究[D]. 昆明: 云南大学硕士学位论文, 2012. YANG Y. Studies on species diversity and thermophilic enzyme from hot spring in part regions of Yunnan[D]. Kunming: Master’s Thesis
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仲秀芳,马景浩,赵萱,袁思涵,富志磊,田淑斌,刘晓艳,范光森. 白酒酿造环境中高温菌株的筛选、鉴定及其特性分析[J]. 微生物学通报, 2025, 52(1): 309-326

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  • 收稿日期:2024-04-10
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