Abstract:The implementation of the Beautiful China construction and the development of new quality productive forces in agriculture necessitate interdisciplinary talents, while the conventional teaching of Microbiology is disconnected from industrial needs and talent training objectives in terms of contents, methods, and assessment. To address the insufficient adaptability of the conventional teaching of this course and construct a teaching model that meets the requirements of talent training in the context of emerging agricultural science. Guided by the concept of outcome-based education (OBE), a “dual-axis four-dimensional” teaching model of collaborative education was constructed. “dual-axis” anchors the ultimate goal of from knowledge to practice. Hierarchical progressive training relying on the “four dimensions” (knowledge impartment, thinking cultivation, ability improvement, and value shaping) is implemented by consolidating the foundation through cloud classrooms and smart classrooms, deepening cognition through exploration of production practice solutions, tempering practical abilities through discipline competitions and skill training, and shaping value through the cultivation of craftsmanship spirit and industry responsibility. In addition, a four-dimensional collaborative teaching platform composed of online cloud classroom, offline smart classroom, experimental project classroom, and enterprise on-site classroom was built. Simultaneously, a dynamic closed-loop improvement mechanism of process-oriented assessment+summative assessment+multi-subject (including teachers and enterprise mentors) assessment was embedded, and cutting-edge biotechnology was deeply integrated with ideological and politic education. The reform practice significantly improved students’ in-depth knowledge mastery, practical innovation ability, and career adaptability. Meanwhile, it effectively enhanced the compatibility of the course with agricultural green development and regional economic development. The constructed “dual-axis four-dimensional” model of collaborative education and supporting reform measures provide a replicable and promotable path for the training of high-quality agricultural talents under the background of emerging agricultural science, highlighting the adaptive value of course reform to industrial needs and talent training objectives.