科微学术

微生物学通报

基于应用场景的微生物组工程化策略
作者:
基金项目:

国家重点研发计划(2021YFC2102200);天津市科技计划(23YFYSHZ00050);天津市合成生物技术创新能力提升行动(TSBICIP-IJCP-001)


Microbiome engineering strategies in different application scenarios
Author:
  • WANG Jingjing

    WANG Jingjing

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • XU Song

    XU Song

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHAO Wei

    ZHAO Wei

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • SHI Liuyang

    SHI Liuyang

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • WANG Xingbiao

    WANG Xingbiao

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • HAN Yifan

    HAN Yifan

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHANG Xiaoxia

    ZHANG Xiaoxia

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • HUANG Zhiyong

    HUANG Zhiyong

    Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [47]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    微生物组在工业、农业、环境、健康等多个领域有巨大的应用潜力,如何构建高效稳定的微生物组,并通过工程化将其应用至各个领域是当前研究的重点和难点。总结分析微生物组的构建策略,以促进微生物组在不同场景下的工程化应用。首先根据应用需求将微生物组的应用场景分为封闭系统、半封闭系统和开放系统,然后回顾了在3种系统中已有的微生物组构建策略,特别分析了其特点和局限,最后根据不同系统的特点,结合实际应用的经验反馈,针对性地提出了微生物组的构建策略。在封闭系统中,主要利用“自下而上”的方法合成微生物组,对微生物个体信息要求较高,对微生物组的效率要求较高;在半封闭和开放系统中,同时利用“自上而下”和“自下而上”的方法构建微生物组,对微生物组的稳定性和安全性要求较高。在不同应用场景中,构建微生物组的策略和侧重点不同,应根据实际情况选择合适的策略构建高效的微生物组。

    Abstract:

    Microbiomes have great application potential in industry, agriculture, environment, health, etc. In practice, it is still difficult to construct efficient and stable microbiomes and utilize the engineered microbiomes in various application fields. To summarize and analyze the construction strategies of microbiomes, thus facilitating the engineering of microbiomes for application in different scenarios. We first classified the application scenarios of microbiomes into closed, semi-closed, and open systems according to the application requirements and then reviewed the existing microbiome construction strategies in the three systems, especially focusing on their characteristics and limitations. Finally, according to the characteristics of different systems and the empirical feedback from practical applications, we proposed the microbiome construction strategies. In the closed system, the microbiome is mainly synthesized by the bottom-up method, which has high requirements for microbial individual information and microbiome efficiency. In semi-closed and open systems, the microbiome is constructed by top-down and bottom-up methods, which require high stability and safety of the microbiome. In diverse application scenarios, the strategies and focuses of constructing microbiomes are different, and appropriate strategies could be chosen to construct efficient microbiomes according to the actual situations.

    参考文献
    [1] BLASER MJ, CARDON ZG, CHO MK, DANGL JL, DONOHUE TJ, GREEN JL, KNIGHT R, MAXON ME, NORTHEN TR, POLLARD KS, BRODIE EL. Toward a predictive understanding of earth’s microbiomes to address 21st century challenges[J]. mBio, 2016, 7(3): e00714-16.
    [2] 刘炜伟, 吴冰, 向梅春, 刘杏忠. 从微生物组到合成功能菌群[J]. 微生物学通报, 2017, 44(4): 881-889. LIU WW, WU B, XIANG MC, LIU XZ. From microbiome to synthetic microbial community[J]. Microbiology China, 2017, 44(4): 881-889(in Chinese).
    [3] AGGARWAL N, KITANO S, PUAH GRY, KITTELMANN S, HWANG IY, CHANG MW. Microbiome and human health: current understanding, engineering, and enabling technologies[J]. Chemical Reviews, 2023, 123(1): 31-72.
    [4] LAWSON CE, HARCOMBE WR, HATZENPICHLER R, LINDEMANN SR, LÖFFLER FE, O’MALLEY MA, GARCÍA MARTÍN H, PFLEGER BF, RASKIN L, VENTURELLI OS, WEISSBRODT DG, NOGUERA DR, McMAHON KD. Common principles and best practices for engineering microbiomes[J]. Nature Reviews Microbiology, 2019, 17(12): 725-741.
    [5] 徐昭勇, 胡海洋, 许平, 唐鸿志. 人工合成微生物组的构建与应用[J]. 合成生物学, 2021, 2(2): 181-193. XU ZY, HU HY, XU P, TANG HZ. Development and application of synthetic microbiome[J]. Synthetic Biology Journal, 2021, 2(2): 181-193(in Chinese).
    [6] CLARK RL, CONNORS BM, STEVENSON DM, HROMADA SE, HAMILTON JJ, AMADOR-NOGUEZ D, VENTURELLI OS. Design of synthetic human gut microbiome assembly and butyrate production[J]. Nature Communications, 2021, 12(1): 3254.
    [7] 刘裕, 韦惠玲, 刘骥翔, 王少杰, 苏海佳. 人工多菌体系的设计与构建: 合成生物学研究新前沿[J]. 合成生物学, 2021, 2(4): 635-650. LIU Y, WEI HL, LIU YX, WANG SJ, SU HJ. Design and progress of synthetic consortia: a new frontier in synthetic biology[J]. Synthetic Biology Journal, 2021, 2(4): 635-650(in Chinese).
    [8] MABWI HA, KIM E, SONG DG, YOON HS, PAN CH, KOMBA EVG, KO G, CHA KH. Synthetic gut microbiome: advances and challenges[J]. Computational and Structural Biotechnology Journal, 2021, 19: 363-371.
    [9] HU HY, WANG MX, HUANG YQ, XU ZY, XU P, NIE Y, TANG HZ. Guided by the principles of microbiome engineering: accomplishments and perspectives for environmental use[J]. mLife, 2022, 1(4): 382-398.
    [10] PAN RZ, YANG XY, QIU M, JIANG WK, ZHANG WM, JIANG YJ, XIN FX, JIANG M. Construction of coculture system containing Escherichia coli with different microbial species for biochemical production[J]. ACS Synthetic Biology, 2023, 12(8): 2208-2216.
    [11] ZHAO CH, SINUMVAYO JP, ZHANG YP, LI Y. Design and development of a “Y-shaped” microbial consortium capable of simultaneously utilizing biomass sugars for efficient production of butanol[J]. Metabolic Engineering, 2019, 55: 111-119.
    [12] ZHOU K, QIAO KJ, EDGAR S, STEPHANOPOULOS G. Distributing a metabolic pathway among a microbial consortium enhances production of natural products[J]. Nature Biotechnology, 2015, 33(4): 377-383.
    [13] JONES JA, VERNACCHIO VR, COLLINS SM, SHIRKE AN, XIU Y, ENGLAENDER JA, CRESS BF, McCUTCHEON CC, LINHARDT RJ, GROSS RA, KOFFAS MAG. Complete biosynthesis of anthocyanins using E. coli polycultures[J]. mBio, 2017, 8(3): e00621-17.
    [14] YAN W, GAO H, JIANG WK, JIANG YJ, LIN CSK, ZHANG WM, XIN FX, JIANG M. The de novo synthesis of 2-phenylethanol from glucose by the synthetic microbial consortium composed of engineered Escherichia coli and Meyerozyma guilliermondii[J]. ACS Synthetic Biology, 2022, 11(12): 4018-4030.
    [15] WEI SY, GAO GR, DING MZ, CAO CY, HOU ZJ, CHENG JS, YUAN YJ. An engineered microbial consortium provides precursors for fengycin production by Bacillus subtilis[J]. Journal of Natural Products, 2024, 87(1): 28-37.
    [16] FENG J, LI RF, ZHANG SS, BU YF, CHEN YC, CUI YL, LIN BX, CHEN YH, TAO Y, WU B. Bioretrosynthesis of functionalized N-heterocycles from glucose via one-pot tandem collaborations of designed microbes[J]. Advanced Science, 2020, 7(17): 2001188.
    [17] de ROY K, MARZORATI M, van den ABBEELE P, van de WIELE T, BOON N. Synthetic microbial ecosystems: an exciting tool to understand and apply microbial communities[J]. Environmental Microbiology, 2014, 16(6): 1472-1481.
    [18] XIAO CQ, CHI R, FANG YJ. Effects of Acidiphilium cryptum on biosolubilization of rock phosphate in the presence of Acidithiobacillus ferrooxidans[J]. Transactions of Nonferrous Metals Society of China, 2013, 23(7): 2153-2159.
    [19] BENOMAR S, RANAVA D, CÁRDENAS ML, TRABLY E, RAFRAFI Y, DUCRET A, HAMELIN J, LOJOU E, STEYER JP, GIUDICI-ORTICONI MT. Nutritional stress induces exchange of cell material and energetic coupling between bacterial species[J]. Nature Communications, 2015, 6: 6283.
    [20] 陈伟, 陈芝, 文莹, 李季伦. 链霉菌遗传不稳定性研究进展与展望[J]. 微生物学报, 2009, 49(10): 1271-1276. CHEN W, CHEN Z, WEN Y, LI JL. Overview and prospect on the genetic instability of Streptomyces: a review[J]. Acta Microbiologica Sinica, 2009, 49(10): 1271-1276(in Chinese).
    [21] HEINKEN A, HERTEL J, ACHARYA G, RAVCHEEV DA, NYGA M, OKPALA OE, HOGAN M, MAGNÚSDÓTTIR S, MARTINELLI F, NAP B, PRECIAT G, EDIRISINGHE JN, HENRY CS, FLEMING RMT, THIELE I. Genome-scale metabolic reconstruction of 7302 human microorganisms for personalized medicine[J]. Nature Biotechnology, 2023, 41(9): 1320-1331.
    [22] ZELEZNIAK A, ANDREJEV S, PONOMAROVA O, MENDE DR, BORK P, PATIL KR. Metabolic dependencies drive species co-occurrence in diverse microbial communities[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(20): 6449-6454.
    [23] CAI JY, TAN TW, CHAN SHJ. Predicting Nash equilibria for microbial metabolic interactions[J]. Bioinformatics, 2021, 36(24): 5649-5655.
    [24] DUKOVSKI I, BAJIĆ D, CHACÓN JM, QUINTIN M, VILA JCC, SULHEIM S, PACHECO AR, BERNSTEIN DB, RIEHL WJ, KOROLEV KS, SANCHEZ A, HARCOMBE WR, SEGRÈ D. A metabolic modeling platform for the computation of microbial ecosystems in time and space (COMETS)[J]. Nature Protocols, 2021, 16(11): 5030-5082.
    [25] CONSORTIUM HMP. Structure, function and diversity of the healthy human microbiome[J]. Nature, 2012, 486(7402): 207-214.
    [26] GENSOLLEN T, IYER SS, KASPER DL, BLUMBERG RS. How colonization by microbiota in early life shapes the immune system[J]. Science, 2016, 352(6285): 539-544.
    [27] LEY RE, BÄCKHED F, TURNBAUGH P, LOZUPONE CA, KNIGHT RD, GORDON JI. Obesity alters gut microbial ecology[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(31): 11070-11075.
    [28] TURNBAUGH PJ, HAMADY M, YATSUNENKO T, CANTAREL BL, DUNCAN A, LEY RE, SOGIN ML, JONES WJ, ROE BA, AFFOURTIT JP, EGHOLM M, HENRISSAT B, HEATH AC, KNIGHT R, GORDON JI. A core gut microbiome in obese and lean twins[J]. Nature, 2009, 457(7228): 480-484.
    [29] LIÉVIN V, PEIFFER I, HUDAULT S, ROCHAT F, BRASSART D, NEESER JR, SERVIN AL. Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity[J]. Gut, 2000, 47(5): 646-652.
    [30] TOURÉ R, KHEADR E, LACROIX C, MORONI O, FLISS I. Production of antibacterial substances by bifidobacterial isolates from infant stool active against Listeria monocytogenes[J]. Journal of Applied Microbiology, 2003, 95(5): 1058-1069.
    [31] CHENG AG, HO PY, ARANDA-DÍAZ A, JAIN S, YU FB, MENG XD, WANG M, IAKIVIAK M, NAGASHIMA K, ZHAO AS, MURUGKAR P, PATIL A, ATABAKHSH K, WEAKLEY A, YAN J, BRUMBAUGH AR, HIGGINBOTTOM S, DIMAS A, SHIVER AL, DEUTSCHBAUER A, et al. Design, construction, and in vivo augmentation of a complex gut microbiome[J]. Cell, 2022, 185(19): 3617-3636.e19.
    [32] GOPALAKRISHNAN V, SPENCER CN, NEZI L, REUBEN A, ANDREWS MC, KARPINETS TV, PRIETO PA, VICENTE D, HOFFMAN K, WEI SC, COGDILL AP, ZHAO L, HUDGENS CW, HUTCHINSON DS, MANZO T, de MACEDO MP, COTECHINI T, KUMAR T, CHEN WS, REDDY SM, et al. Gut microbiome modulates response to anti-PD-1 immunotherapy in melanoma patients[J]. Science, 2018, 359(6371): 97-103.
    [33] BUFFIE CG, BUCCI V, STEIN RR, McKENNEY PT, LING LL, GOBOURNE A, NO D, LIU H, KINNEBREW M, VIALE A, LITTMANN E, van den BRINK MRM, JENQ RR, TAUR Y, SANDER C, CROSS JR, TOUSSAINT NC, XAVIER JB, PAMER EG. Precision microbiome reconstitution restores bile acid mediated resistance to Clostridium difficile[J]. Nature, 2015, 517(7533): 205-208.
    [34] 中华医学会肠外肠内营养学分会, 中国国际医疗保健促进交流会加速康复外科分会, 中国微生态治疗创新联盟, 上海预防医学会微生态专业委员会. 菌群移植标准化方法学的建立与临床应用中国专家共识[J]. 中华胃肠外科杂志, 2020, 23(S1): 5-13. Chinese Society for Parenteral and Enteral Nutrition, China International Healthcare Promotion and Exchange Association Accelerated Rehabilitation Surgery Branch, China Microecological Therapy Innovation Alliance, Microecology Professional Committee of Shanghai Preventive Medicine Association. Chinese experts consensus on standardized methodology and clinical application of fecal microbiota transplantation[J]. Chinese Journal of Gastrointestinal Surgery, 2020, 23(S1): 5-13(in Chinese).
    [35] ZUFFA S, SCHMID R, BAUERMEISTER A, GOMES PWP, CARABALLO-RODRIGUEZ AM, EL ABIEAD Y, ARON AT, GENTRY EC, ZEMLIN J, MEEHAN MJ, AVALON NE, CICHEWICZ RH, BUZUN E, TERRAZAS MC, HSU CY, OLES R, AYALA AV, ZHAO JQ, CHU H, KUIJPERS MCM, et al. microbeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data[J]. Nature Microbiology, 2024, 9(2): 336-345.
    [36] BEGUM N, WANG L, AHMAD H, AKHTAR K, ROY R, KHAN MI, ZHAO TJ. Co-inoculation of arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria improve growth and photosynthesis in tobacco under drought stress by up-regulating antioxidant and mineral nutrition metabolism[J]. Microbial Ecology, 2022, 83(4): 971-988.
    [37] ZHOU X, WANG JT, LIU F, LIANG JM, ZHAO P, TSUI CKM, CAI L. Cross-Kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease[J]. Nature Communications, 2022, 13(1): 7890.
    [38] HE Y, YUN H, PENG L, JI J, WANG WX, LI XK. Deciphering the potential role of quorum quenching in efficient aerobic denitrification driven by a synthetic microbial community[J]. Water Research, 2024, 251: 121162.
    [39] WANG JJ, ZHAO SQ, XU S, ZHAO W, ZHANG XX, LEI Y, ZHAI HH, HUANG ZY. Co-inoculation of antagonistic Bacillus velezensis FH-1 and Brevundimonas diminuta NYM3 promotes rice growth by regulating the structure and nitrification function of rhizosphere microbiome[J]. Frontiers in Microbiology, 2023, 14: 1101773.
    [40] LI PF, DINI-ANDREOTE F, JIANG JD. Exploiting microbial competition to promote plant health[J]. Trends in Plant Science, 2024, 29(10): 1056-1058.
    [41] XU XH, ZARECKI R, MEDINA S, OFAIM S, LIU XW, CHEN C, HU SL, BROM D, GAT D, POROB S, EIZENBERG H, RONEN Z, JIANG JD, FREILICH S. Modeling microbial communities from atrazine contaminated soils promotes the development of biostimulation solutions[J]. The ISME Journal, 2019, 13(2): 494-508.
    [42] LIN ZY, ZHOU J, HE L, HE XJ, PAN ZL, WANG YM, HE Q. High-temperature biofilm system based on heterotrophic nitrification and aerobic denitrification treating high-strength ammonia wastewater: nitrogen removal performances and temperature-regulated metabolic pathways[J]. Bioresource Technology, 2022, 344: 126184.
    [43] 朋婷婷, 项兴佳, 冯有智, 何世颖, 吴宇澄, 张锋, 曾军, 林先贵. 磁性纳米颗粒介导分离技术筛选土壤中多氯联苯降解菌及其降解特性[J]. 微生物学通报, 2023, 50(9): 3771-3783. PENG TT, XIANG XJ, FENG YZ, HE SY, WU YC, ZHANG F, ZENG J, LIN XG. Magnetic nanoparticle-mediated isolation and degradation characterization of a PCB-degrading strain[J]. Microbiology China, 2023, 50(9): 3771-3783(in Chinese).
    [44] PANKE-BUISSE K, POOLE AC, GOODRICH JK, LEY RE, KAO-KNIFFIN J. Selection on soil microbiomes reveals reproducible impacts on plant function[J]. The ISME Journal, 2015, 9(4): 980-989.
    [45] RUAN ZP, CHEN K, CAO WM, MENG L, YANG BG, XU MJ, XING YW, LI PF, FREILICH S, CHEN C, GAO YZ, JIANG JD, XU XH. Engineering natural microbiomes toward enhanced bioremediation by microbiome modeling[J]. Nature Communications, 2024, 15(1): 4694.
    [46] RAWAT P, SHARMA A, SHANKHDHAR D, SHANKHDHAR SC. Improvement of phosphorus uptake, phosphorus use efficiency, and grain yield of upland rice (Oryza sativa L.) in response to phosphate- solubilizing bacteria blended with phosphorus fertilizer[J]. Pedosphere, 2022, 32(5): 752-763.
    [47] GILMORE SP, LANKIEWICZ TS, WILKEN SE, BROWN JL, SEXTON JA, HENSKE JK, THEODOROU MK, VALENTINE DL, O’MALLEY MA. Top-down enrichment guides in formation of synthetic microbial consortia for biomass degradation[J]. ACS Synthetic Biology, 2019, 8(9): 2174-2185.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

王敬敬,徐松,赵维,侍浏洋,王兴彪,韩一凡,张小霞,黄志勇. 基于应用场景的微生物组工程化策略[J]. 微生物学通报, 2024, 51(12): 4909-4921

复制
分享
文章指标
  • 点击次数:78
  • 下载次数: 156
  • HTML阅读次数: 179
  • 引用次数: 0
历史
  • 收稿日期:2024-06-26
  • 录用日期:2024-08-25
  • 在线发布日期: 2024-12-24
  • 出版日期: 2024-12-20
文章二维码