水体病毒钙离子絮凝浓缩新方法研究

Studies on Calcium Ion Flocculation of Viruses from Water Samples

摘要:

研究建立了一种从水体中浓缩病毒的新方法，即钙离子絮凝-柠檬酸缓冲液洗溶法，该方法的要点是先用一定量的钙离子溶液和钙离子絮凝剂絮凝水体中的病毒，再用pH 5.0的0.3 mol/L的柠檬酸缓冲液洗溶,然后再进一步超滤浓缩。此法可方便地将水体中的病毒浓缩10000倍以上。应用该法分别对人工播种于饮用水中的f₂噬菌体和脊髓灰质炎疫苗病毒(PV₁)进行了浓缩，结果发现f₂噬菌体的平均回收率达96%，而PV₁的回收率为100%，均显著高于阳电膜过滤法(P<0.05)。该方法快速、简便、有效。

Abstract:

A new procedure for virus concentration from water samples has been developed. This method called calcium flocculation-citrate buffer method involves virus flocculation formed by 1 mol/L Ca²⁺ solution and its flocculating agent, virus release with sodium citrate dissolution (0.3 mol/L, pH5.0), and virus reconcentration by ultrafiltration. It can concentrate the viruses 10000 times easily. Seeding experiments showed that it could recover f₂ phage in an average rate of 96%, and 100% for the vaccine poliovirus type 1 (PV₁), which was significant higher than the current positive charged filter method (P<0.05). The established approach is rapid, simple and effective.