用DGGE技术分析污水人工快速渗滤系统中微生物种群分布

Analysis of Microbial Community Distribution in Constructed Rapid Infiltrition System(CRI) by DGGE

摘要:

从深圳运行中的人工快速渗滤系统(CRI)的砂层填料中以5cm～10cm间隔垂直取10个样品，进行16S rDNA V3区的DGGE分析，结果表明，CRI系统中微生物种群随着深度的增加逐渐减少，在快渗池砂层填料的30cm深度范围内，微生物群落组成至少有18种，主要是Firmicutes、alpha proteobacterium中的异养菌，它们是COD降解的主要参与者；而在40cm及更深范围，菌群减少为12种甚至更少，优势菌是Acidovorax sp.、Nitrospira sp.、Clostridium sp.等，表明快渗池下部存在较强的硝化作用和厌氧的微环境，快渗池上、下层之间呈现出不同的多样性。结果揭示出CRI系统中的微生物种群空间分布状况，为其处理效果的稳定和提高提供了理论基础。

Abstract:

Sands of different depth were sampled from Constructed Rapid Infiltrition system(CRI) in Shenzhen, and they were analyzed by Denaturing Gradient Gel Electrophoresis(DGGE). The profile of DGGE showed that, the microbial community decreased from top to bottom and in the upper 30cm part, CRI was rich in microbial community about 18 species which played some important roles in the biodegradation of COD, such as Firmicutes、alpha proteobacterium and so on; but less than 12 species existed below 40cm, such as Acidovorax sp.、Nitrospira sp.、Clostridium sp., this indicated that there were some nitrification phenomenon and anaerobic microenvironment in the lower part of CRI. The microbial diversity was prominent different spatially. The results showed the microbial community distribution and were certain meaningful to the stabilization and improvement of CRI.