植酸酶的纯化及其酶学性质初步研究

Studies on Purification and Properties of Phytase from Trichoderma viride

摘要:

从绿色木霉LH374固态发酵产物中提取植酸酶。粗酶液经硫酸铵沉淀、凝胶过滤、离子交换层析纯化后，提纯倍数可达13.3，回收率为27.1%。酶学性质研究表明，植酸酶最适作用温度为55℃、最适作用pH为6.0，米氏常数Km为0.15mmol/L。

Abstract:

The phytase was extracted from solid state leavening of Trichoderma Viride LH374. The crude product was purified by (NH₄)₂SO₄ precipitation, gel filtration and ion exchange chromatography. The purified phyatse was 13.3 times of the raw products, and the extraction ration was 27.1%. The study on the enzymology of phyatse showed that the optimal temperature and pH were 55℃ and 6.0, respectively. The Km value of the phytase was 0.15mmol/L.