两株耐碱酵母的pH耐受实验观察

Examination of pH Tolerance Test of Two Alkalitolerant Yeasts

摘要:

对分离鉴定的两株酵母菌用比浊法和Bradford法进行pH耐受范围检测。Trichosporon asahii XJU-1的pH耐受范围为2.0～13.0，最适pH值为8.0。Rhodotorula mucilaginosa XJU-1的pH耐受范围为3.0～12.0，最适pH值为8.5。比浊法在pH4.0～10.0测出数值比较可靠，而在pH<4.0和pH≥10.0产生了较大误差。在最适pH时，Bradford法间接测定两酵母的生长量，T. asahii总溶解蛋白为290μg/mL，Rh. mucilaginosa总溶解蛋白为164μg/mL。Bradford法在pH2.0～13.0范围均能较准确地反映出菌株生长状况，数据表明两株酵母有广阔的pH耐受性，它们是耐碱酵母菌的新成员。

Abstract:

The purpose of this paper is to examine the pH adaptability range of two yeasts from our laboratory, and applied turbidimetry and Bradford methods to examine growth of Trichosporon asahii XJU-1 and Rhodotorula mucilaginosa XJU-1.It is shown that Trichosporon asahii XJU-1 grown between pH2.0 and pH13.0 and optimum pH is 8.0, whereas Rhodotorula mucilaginosa XJU-1 grown between pH3.0 and 12.0，optimum pH is 8.5. When turbidimetry was applied, it produced consensus results between pH4.0 and 10.0 with Bradford method. At the same time, produced senior distorted at pH<4.0 and pH≥10.0.Bradford method indirect determined total soluble protein of Trichosporon asahii XJU-1 is 290μg/mL at optimum pH8.0, Rhodotorula mucilaginosa XJU-1 is 164μg/mL at optimum pH8.5, respectively. Compared with turbidimetry, Bradford method embodied its accuracy in whole pH ranges. It is concluded that both two tested yeasts have wider pH tolerant ranges and can be two new members of alkali-tolerant yeast family.