维吉霉素产生菌株X-435的诱变育种

Studies on Mutagenesis of Virginiamycin Producing Strain X-435

摘要:

链霉菌菌株x-435是从北京郊区采集的土样中分离得到的1株维吉霉素产生菌。为了提高维吉霉素的产量，采用紫外线对出发菌株x-435进行紫外线诱变处理，诱变处理后在高氏培养基上产生3种菌落形态，确定草帽型的菌落为阳性突变株的菌落形态，经过筛选获得效价高于出发菌株近20倍的突变株F5-25-u-28，且传代5代后稳定性较好。

Abstract:

Streptomyces sp. X-435 isolated from a soil sample collected in the suburbs of Beijing was proved to be a produce Virginiamycin. To improve the productivity of the Virginiamycin of Streptomyces sp. X-435,the spores of strain X-435 were treated with UV. The three types of colony,strawhat, wrinkled, blad, were isolated on Gaose's medium plates after mutation. Among them, the colonies of strawhat type exhibited positive mutation and were picked up as objects of screening. After five generation of mutation, the mutant F5-25-u-28 was selected which potency of Virginiamycin was about 20times higher than that of the beginning strain by flask fermentation and was also genetic stable.