糙皮侧耳担孢子交配型的鉴定

Accurate Determination of Mating Type of Basidispores in Pleurotus ostreatus

摘要:

以3个糙皮侧耳菌株为材料，用核迁移试验和OWE-SOJ技术对担孢子的4种交配型进行了鉴定。核迁移试验表明，核迁移仅出现于A＝B≠交配反应而不出现于A≠B＝反应中。而在OWE-SOJ试验中，发生核迁移的A＝B≠交配反应形成无锁状联合的绒毛状菌落，而不发生核迁移的A≠B＝反应形成无锁状联合的栅栏型菌落，两种菌落可以清楚地区别开来。核迁移试验的结果与OWE-SOJ试验的结果是一致的，可以相互印证。因此，将两者用于糙皮侧耳交配型的准确鉴定是可行的。

Abstract:

Four mating types of basidispores in 3 strains of Pleurotus ostreatus were determined using a nuclear migration test and an OWE-SOJ technique. The nuclear migration test showed that nuclear migration only occurred in A=B≠ mating interaction but did not in A≠B= interaction. In OWE-SOJ test, a fluffy colony without clamp connection formed in the A=B≠ mating interaction where nuclear migration occurs, while a barrage colony without clamp connection formed in A≠B= interaction where nuclear migration do not occur. The two colonies could be distinguished clearly each other. The result of the nuclear migration test is consistent with that of OWE-SOJ, which could be corroborated each other. Therefore it is feasible to apply the two to accurate determination of mating types in Pleurotus ostreatus.