莱氏野村菌产几丁质酶条件及酶学性质研究

Studies on the Production and Properties of Chitinase from Nomuraea rileyi Strain CQ031021

摘要:

对莱氏野村菌(Nomuraea rileyi)菌株CQ031021产几丁质酶条件及酶学性质进行了研究。结果表明：该菌株最适产酶碳源为2.0%(W/V)葡萄糖，氮源为1.2%(W/V)复合氮源(蛋白胨、牛肉膏按1∶1的比例)，接种量为孢悬液2mL(1×10⁷个/mL)，培养温度28℃，培养液初始pH6.0，培养时间6d；一定浓度的吐温-80对几丁质酶活性有促进作用，而SDS有抑制作用；粗酶液最适反应温度50℃，最适pH6.0，在40℃以下及pH5.5～6.5范围内酶活力较稳定。

Abstract:

The producing condition and properties of chitinase secreted by Nomuraea rileyi strain CQ031021 were studied. The optimal conditions for the strain to produce chitinase are 6 days of 28℃ with the initial pH 6.0, and the liquid medium containing 2.0% glucose as its carbon source and 0.6% peptone plus 0.6% beef extract as nitrogen source after inoculating dosage 2mL suspension of conidia (1×10⁷/mL). The optimal temperature and pH for enzyme activity is 50℃ and 6.0, respectively, while the activity can be enhanced by Tween-80 and inhibited by SDS. The enzyme activity is stable under 40℃ and in pH range of 5.5～6.5.