转基因棉花根际土壤DNA的提取方法研究

Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton

摘要:

采用直接裂解方式，通过玻璃珠、溶菌酶和SDS共同作用直接从转基因棉花SGK321和中棉所41的根际土壤中提取微生物DNA。结果表明，该方法能从两种转基因棉花根际土壤中提取到20kb大小的完整的DNA片段。所得DNA完全适用于PCR扩增和酶切的要求。

Abstract:

A direct lysis manner for extracting DNA directly from rhizosphere soil of transgenic cotton SGK321 and Zhongmiansuo 41 was designed by glass bead-lysozyme-SDS method. The results showed that intact DNA fragments over 20 kb were gained from rhizosphere soil planted two cultivars of transgenic cotton. DNA extracted suited to PCR amplification and RFLP.