一株分离于酸性矿坑水的异养菌的特性与分子鉴定

Characteristics and Molecular Identification of a Strain of Heterotrophic Microorganism Isolated from Acid Mine Drainage

摘要:

采用平板划线分离法，从江西某铜矿酸性矿坑水中分离出一株极端嗜酸、又能耐受中度碱性条件的异养微生物，命名为HJM菌株。该菌株能在pH1.5～10.0的范围内生长。形态学以及18S rDNA和26S rDNA D1/D2区序列分析表明，HJM菌株属于P.guilliermondii这个种。金属抗性试验表明，该菌株对重金属铜离子的抗性可高达45mmol/L，因此，它的分离为研究极端环境中微生物的抗铜机制提供了材料。

Abstract:

By streak plate method, a strain of heterotrophic microorganism which was extremely acidophilic, alkalitolerant, named by HJM, was isolated from an acid mine drainage of a certain copper mineral in Jiangxi Province. This strain was able to grow under pH value of 1.5～10.0. Morphological characteristics and sequences analysis of 16S rDNA and 26S rDNA D1/D2 region revealed that it belonged to the species P.guilliermondii. Resistant experiment to metals showed that it could resist copper of 45mmol/L, so it was an important strain used to study the resistance mechanism of copper.