链孢粘帚霉几丁质酶的诱导及其抗真菌活性研究

Chitinases Induced from Gliocladium catenulatum HL-1-1 and Their Antagonistic Activity Against Plant Pathogenic Fungi

摘要:

链孢粘帚霉HL-1-1菌株在不同培养条件下产几丁质酶活性不同，核盘菌菌核对其几丁质酶的诱导作用高于几丁质；首次用菌核配制培养基成功诱导了几丁质酶的产生，其产酶的最适初始pH值为4.5，最适培养时间为6d。几丁质酶对10种病原菌都有不同的抑制作用，对小麦雪腐病、葡萄白腐病、玉米黄斑病及斑点落叶病等病原菌的孢子萌发具有明显的抑制作用；该几丁质酶还能明显抑制核盘菌和立枯丝核菌的菌核萌发。

Abstract:

Induction and antagonistic activity of chitinases produced by Gliocladium catenulatum HL-1-1 were studied. Results showed that the isolate could produce chitinases under various culture conditions. Sclerotia powder could induce the isolate to produce much more chitinases. The optimum conditions to produce the enzyme were pH 4.5 at initial medium and to culture the isolate for 6 days. The chitinases were proved to inhibit the conidial germination of Fusarium nivale, Coniella diplodiella, Curvularia lunata and Alternaria alternata and the sclerotial germination of Sclerotinia sclerotiorum and Rhizoctonia solani obviously.