抗感青枯病番茄的内生细菌数量动态分析及其对青枯病的生物防治

The Dynamic of Endophytic Bacteria at Different Growth Stage of Tomato and Biological Control of Tomato Bacterial Wilt

摘要:

对番茄内生细菌数量动态及其对青枯病的生物防治研究结果表明：番茄内生细菌可来源于种子内部。番茄不同生育期，内生细菌数量最多在成株期，其中抗病品种根、茎分别为24.3×10⁴CFU/g鲜重和22.9×10⁴ CFU/ g鲜重,感病品种根、茎分别为9.8×10⁴CFU/g鲜重和13.4×10⁴CFU/g鲜重。抗病品种中具有拮抗青枯菌的内生细菌菌株为17个,感病品种中7个。部分内生细菌具促进番茄种子萌发和防治番茄青枯病的作用，其中5R和3R内生菌株的防病效果分别达91.7%和81.3%。

Abstract:

The dynamic of endophytic bacteria at different growth stage of tomato and use of these endophytic bacteria to control tomato bacterial wilt were studied. The results showed that endophytic bacteria could be found in the tomato seeds and their quantities reached the highest peak in the adult plants both in resistant and susceptible cultivars. The amount of endophytic bacteria in adult plants of resistant tomato cultivars was 2.43×10⁵CFU/g FW in the root and 22.9×10⁴ CFU/g FW in the stem, while the amount of endophytic bacteria in adult plants of susceptible tomato cultivars was 9.8×10⁴CFU/g FW in the root and 13.4×10⁴CFU/g FW in the stem respectively. Seventeen strains of endophytic bacteria from resistant cultivars and only seven strains from susceptible cultivars were found to be antagonistic to Ralstonia solanacearum. In addition, some strains of endophytic bacteria had the abilities of promoting tomato seed germination and controlling tomato bacterial wilt, among which, strain 5R and 3R had better control effect of 91.7% and 81.3% respectively.