银耳二型态细胞差异性的初步研究

Preliminary Study on the Differences of Dimorphic Cells in Tremella fuciformis

摘要:

以银耳的担孢子、节孢子和菌丝体为材料，通过显微观察和电泳分析揭示银耳二型态细胞间的差异性。显微形态及核相观察表明：节孢子直径略大于担孢子，担孢子为单核细胞，节孢子绝大多数为单核细胞，少数为双核细胞，菌丝体为双核细胞并具有典型的锁状联合结构。总蛋白及同工酶电泳分析表明：菌丝相总蛋白谱带多于酵母相，过氧化物及酯酶与酵母相存在一定差异，而多酚氧化酶基本相同。因此，伴随银耳二型态细胞两相形态的转变，细胞代谢水平发生了相应变化。

Abstract:

In order to reveal the differences among the dimorphic cells, basidiospores, arthrospores and mycelia of Tremella fuciformis were subjected to microscopical observation and electrophoresis analysis. Morphology and nucleus phase showed that arthrospores are larger than basidiospores in diameter, basidiospores are mononuclear cells, the majority of arthrospores are dikaryocytes and the mononuclear cells are in minority, mycelia are dikaryocytes with typical clamp connection. The whole protein and isozyme analysis indicated that the mycelial form contains more bands than that of yeast form and some differences in peroxidase and esterase between the two forms were found, while the polyphenol oxidase are almost similar. Therefore, corresponding changes in metabolism level are occurred during the form transition of the dimorphic cell in T. fuciformis.