红树林内生海洋细菌CⅢ-1菌株胞外代谢产物GC/MS分析

GC/MS Analysis of the Extracellular Metabolism of the Endophytic Marine Bacterium CⅢ-1 from Mangrove

摘要:

红树林内生海洋细菌CⅢ-1菌株胞外代谢产物的二氯甲烷萃取物GC/MS分析结果表明，该菌株胞外代谢产物的二氯甲烷萃取物的主要化学成分及相对含量为：花生酸（10.12%）、角鲨烯（7.33%）、12-二十三酮（7.07%）、2-(2-羟基乙氧基)乙基硬脂酸酯（6.21%）、四甲基环氧乙烷（4.67%）、吡咯-2-乙酸,4-(1-氯-1-癸烯基)-3,5-二甲基乙基酯（4.63%）、3-甲基-3-十一碳烯（3.55%）等。其中角鲨烯具有重要的应用价值。

关键词:海洋细菌，胞外代谢产物，气相色谱/质谱法，分析

Abstract:

The extracellular metabolism products of the marine bacterium CⅢ-1, a endophytic marine bacterium isolated from mangrove (V.C Bruguiera gymnorrhiza), were extracted with dichloromethane and then analyzed by GC/MS technique. The results showed that the mainly chemical composition and their concentrations of the extracellular metabolism of this strain were arachic acid（10.12%）, all-trans-squalene（7.33%），12-tricosanone（7.07%），2-(2-hydroxyethoxy)ethyl stearate（6.21%），tetramethyl-oxirane（4.67%）, pyrrole-2-carboxylicacid-4-(1-chlorodec-1-enyl)-3,5-dimethyl-ethylester(4.63%）, 3-methyl-3-undecene（3.55%） and so on. Among them the all-trans-squalene has an important value of application.

Key words:Marine bacterium, Extracellular metabolism, GC/MC, Analysis