新疆胀果甘草内生菌的分离和鉴定

Isolation and Characterization of Endophytic Microorganisms in Glaycyrrhiza inflat Bat. from Xinjiang

摘要:

对采自新疆的健康野生胀果甘草不同组织中的内生菌进行分离,确立了分离的条件为5% NaClO₄浸5min，分离纯化得到149株细菌和2种真菌。通过形态学观察和革兰氏染色，细菌中芽孢杆菌为93株，杆状菌56株，使用法国梅里埃细菌自动鉴定仪对其进行鉴定，得到鉴定结果的细菌属于13个属,真菌显微形态鉴定属于Penicillium青霉菌属和Fusarium镰刀菌属。

Abstract:

The present investigation was undertaken in order to select the surface-sterilization technique most efficient for eliminating epiphytes, to document endophytes of healthy tissues from Glaycyrrhiza inflat Bat. in Xinjiang. Surface sterilization with 5% commercial solution of sodium hypochlorite for 5 minutes was reaffirmed as adequate for removing epiphytes on licorice roots. From the 151 segments incubated, 149 bacterial isolates and 2 fungal isolates were obtained. From all the isolates, Bacterial isolates were identified by VITEK-AMS. Part of Bacteria were identified in 13 different genus. Fungal species were characterized as Penicillium sp. and Fusarium sp.with microscope.