氨基糖苷类抗生素JI-20A发酵过程的磷酸盐控制

Control of Phosphate Concentration on Aminoglycoside Antibiotic JI-20A Fermentation

摘要:

磷酸盐初始浓度6.1mmol/L～9.6 mmol/L有助于菌体的生长，但抑制氨基糖苷类抗生素JI-20A的合成。在产物合成期磷酸盐浓度控制在1.1 mmol/L以下可提高碱性磷酸酯酶活力，降低丙酮酸浓度，促进氨基糖苷类抗生素JI-20A合成。

Abstract:

It was found that the phosphate concentration effected significantly on the cell growth and the production of aminoglycoside antibiotic JI-20A. Although initial phosphate concentration of 6.1mmol/L～9.6 mmol/L was beneficial for the cell growth, the production of aminoglycoside antibiotic JI-20A was inhibited. When the phosphate concentration was controlled below 1.1 mmol/L in the biosynthesis phase of aminoglycoside antibiotic JI-20A, high alkaline phosphatase activity and low pyruvic acid concentration were resulted in, and the production of aminoglycoside antibiotic JI-20A was enhanced.