复合诱变和抗性筛选利迪链菌素高产菌株

Breeding of High Streptolydigin-Producing Strain by Complex Mutagenesis and Resistance Selection

摘要:

为解决利迪链霉菌生产能力低的问题，以Streptomyces lydicus AS 4.2501-P28为出发菌株，应用紫外和吖啶橙诱变，结合抗生素的抗性筛选，得到稳定高产的S. lydicus AS 4.2501-L8，发酵水平达到177.0 μg/mL，较出发菌株提高了96.2%。

Abstract:

A high Streptolydigin-producing strain Streptomyces lydicus AS 4.2501-L8 with genetic stability was obtained from the original strain S. lydicus AS 4.2501-P28 by mutagenesis with UV and acridine orange, followed by resistance selection. Its yield of Streptolydigin was 177.0 μg/mL, 96.2% higher than the original strain.