海水中DHA产生菌的筛选及一株高产菌的鉴定

Screening of the Microbe Producing Docosahexaenoic Acid from Seawater and Identification on a Strain of High Yield

摘要:

从海水中筛选产DHA的微生物，共采集海水样品280余份，用苏丹黑染色法得到160株产油脂菌株，在对60株脂肪粒较大的微生物用索氏抽提法提取油脂后，初筛得到油脂含量高于8%的菌株7株。对10株油脂产量较高的菌株进行复筛，编号7-3的菌株油脂含量达到15.9％，DHA在油脂中的含量达到45.2%，选用7-3作为目的菌株。对7-3进行形态特征、培养特征及生理生化特征鉴定，初步判定菌株7-3为酒香酵母属(Brettanomyces sp.)。

Abstract:

Microbes that produce Docosahexaenoic Acid were isolated from seawater. 160 strains capable of producing lipids were screened out using Sudan Black B dying method from 280 seawater samples. From 60 strains of microorganisms producing bigger lipid particles, 7 strains of them capable of producing lipids more than 8% were obtained with Soxhlet abstracting method in the first screening. In the secondary screening from 10 strains with high lipids yield, strain 7-3 capable of producing 15.9% lipids was obtained, in which the content of DHA（Docosahexaenoic Acid）is 45.2%. Strain 7-3 was identified as Brettanomyces based on its morphological properties, cultural characteristics, physiological and biochemical properties.