广东中山地区气单胞菌环境株的分离、鉴定及系统发生关系

Isolation, Identification and Phylogenetic Relationship of Environmental Strains of Aeromonas spp. from Zhongshan Area, Guangdong Province

摘要:

从广东省中山市的池塘水样、底泥、健康鱼、肠道及稻田土样中用Aeromonas的选择培养基分离到10株气单胞菌。通过生理生化测试、16S rDNA序列测定、与气单胞菌典型菌株的16S rDNA序列进行比对和聚类分析，对它们进行了鉴定，并研究了它们之间的系统发生关系。结果显示该地区环境中气单胞菌的优势种除A. hydrophila（HG1组）外, 还有A. caviae（HG4组）、A. jandaei(HG9组)和A. veronii(HG10组)，其中后两种是国内新记录。这是国内首次对环境中气单胞菌多样性进行研究。

Abstract:

Ten strains of Aeromonas spp. using a selective media Aeromonas agar base were isolated from the water, sediment and intestine content of healthy fish in fish ponds as well as the mud sample taken from rice fields, in Zhongshan city, Guangdong Province. They were identified by means of conventional physiological and biochemical tests together with 16S rDNA sequencing. In addition, the phylogenetic relationship between these strains and some type strains of Aeromonas spp. was analyzed based on their 16S rDNA sequences. Aeromonas hydrophila was found to be the most frequently isolated Aeromonas species as expected. Other isolates were identified as Aeromonas caviae, A. jandaei, A. veronii, respectively, among which A. jandaei and A. veronii were new record of Aeromonas species in China. This is the first study for the diversity of Aeromonas species in the environment in our country.