聚-ε-赖氨酸高产菌株的筛选及其发酵工艺的初步研究

Screening of Poly-ε-lysine-producing Strain and its Fermentation Technology

摘要:

利用亚甲基蓝平板初筛、摇瓶复筛，从土壤中筛选了一株聚-ε-赖氨酸（ε-PL）高产菌株，ε-PL摇瓶产量大于2g/L，经初步鉴定该菌株为白色链霉菌（Streptomyces albulus）。对该菌株发酵生产ε-PL的培养基成分进行初步研究表明：葡萄糖是最好的碳源，酵母粉和硫酸铵是最佳的复合氮源，在优化培养基下，ε-PL摇瓶产量达到3.9 g/L。

Abstract:

A strain with 2 g/L yield of poly-ε-lysine was firstly screened from soil on an agar plate containing methylene blue and then further selected by a flask experiment. The strain was preliminarily identified as Streptomyces albulus. The experiment about medium optimization showed that glucose was the best carbon sources and yeast extract and (NH₄)₂SO₄ were the best compound nitrogen sources. The yield of ε-PL reached 3.9 g/L with optimal medium.