嗜热乙醇杆菌中醛/醇脱氢酶的双启动子分析

The Promoter Analysis of the adhE Gene Encoding the Aldehyde/alcohol Dehydrogenase in Thermoanaerobacter ethanolicus

摘要:

克隆了嗜热乙醇杆菌（Thermoanaerobacter ethanolicus）中乙醇代谢的关键酶之一醛/醇脱氢酶（alcohol/acetaldehyde dehydrogenase, AdhE）基因的上游假定启动子序列，并进行了结构分析。结果表明，adhE的上游序列是启动子，能启动报告基因在大肠杆菌中持续表达。首次发现adhE的启动子序列中存在两个独立的启动子（P₁₇₂和P₃₇）和核糖体结合位点（SD₁₇₂和SD₃₇），分别都具有完整功能，但其活性均低于完整的启动子序列。由此推测嗜热乙醇杆菌中adhE的表达受这两个启动子协同调控。

Abstract:

The natural promoter of aldehyde/alcohol dehydrogenase (AdhE) gene (adhE) from Thermoanaerobacter ethanolicus was analyzed in Escherichia coli using a gene expression reporter system. The sequence immediately upstream of adhE is recognized as a constitutive promoter in E.coli, as well as being presumably an active promoter in T.ethanolicus. The natural promoter of adhE is composed of two independent promoter regions, although activities of the two independent promoter regions are much lower than that of the natural promoter. The results indicate that the AdhE synthesis is cooperatively regulated by the two promoters in T.ethanolicus.