地衣芽孢杆菌16S rRNA基因的TD-PCR扩增及系统发育分析

The TD-PCR and Phylogenetic Analysis of Bacillus licheniformis 16S rDNA

摘要:

运用16S rRNA基因序列分析了中国工业微生物菌种保藏管理中心（CICC）保存的30株地衣芽孢杆菌的系统发育关系，结果显示：24株菌株位于地衣芽孢杆菌系统发育分支；3株菌株位于蜡状芽孢杆菌－苏云金芽孢杆菌系统发育分支；1株菌株位于枯草芽孢杆菌系统发育分支；2株菌株与其它地衣芽孢杆菌菌株间序列同源性为96.4%～97.4%，明显低于其它地衣芽孢杆菌菌株间同源性，分类地位不明确，有待进一步讨论。通过比较分析16S rRNA基因5′端500bp、3′端500bp以及其全基因的系统发育树，表明16S rRNA基因5′端500 bp可以很好的代表全基因序列进行系统发育研究，可用于区分地衣芽孢杆菌、枯草芽孢杆菌以及蜡状芽孢杆菌分支。

Abstract:

16S rDNA sequences of 30 Bacillus strains originally identified as Bacillus licheniformis from China Center of Industrial Culture Collection (CICC) were determined and analyzed. The results indicated that 24 strains are affiliated to Bacillus licheniformis；3 strains are affiliated to Bacillus cereus and 1 strain is affiliated to Bacillus subitilis；the similarity levels of 16S rDNA among the rest of 2 strains and other strains of Bacillus licheniformis，range from 96.4% to 97.4%，further tests are needed to clarify their position. Also we testified that 5' terminal 500bp of 16S rDNA is available to differentiate the strains of Bacillus licheniformis、Bacillus subtilis and Bacillus cereus.