海洋细菌E18菌株的生物学特性及其蓝紫色素稳定性的研究

Biological Characteristics of Marine Bacterium Strain E18 and the Stability of its Indigo Pigment

摘要:

从海泥中分离获得一株海洋细菌Ｅ18菌株,发现其具有稳定产生蓝紫色素的特性。对该菌的形态特征、培养特征及生理生化特征进行了研究。对该菌的分子鉴定结果表明，该菌为假交替单胞菌属细菌。萃取该菌的色素，并试验光、紫外线、热、pH、氧化剂及还原剂对该色素的影响，结果表明：该色素的最大吸收峰为579nm，紫外光对其稳定性无影响，但自然光对其有一定的消色作用。60℃～80℃温度范围对色素有一定的增色作用，而90℃以上高温可使色素消色。色素在pH3～9区域内稳定。色素对还原剂Na₂SO₃较为稳定, 而高浓度的氧化剂H₂O₂可使色素改变颜色。

Abstract:

One strain of Pseudoalteromonas sp. E18 was isolated from the sea mud of Ningbo, Zhejiang. It can produce indigo pigment. The morphological, cultural and biochemical characteristics of the bacterium were studied. The indigo pigment was also extracted. The results showed that the maximum absorption peak of the pigment was at 579nm. The pigment was stable to UV, Na₂SO₃，It was also stable at pH 3～9. The pigment was unstable to the sunlight and high concentration H₂O₂. Temperature of 60℃～80℃ could increase the hue while temperature higher than 90℃ could reduce the hue.