异常球菌007T形态和辐射抗性的研究

Study on Morphology and Radiation Resistance of Deinococcus 007T

摘要:

观察耐辐射异常球菌Deinococcus属的模式菌株Deinococcus radiodurans R1(AS1.633)和野生型异常球菌007^T的生长特性和形态特征；与耐辐射模式菌株AS1.633和辐射敏感菌株 Escherichia coli DH5α 的进行平行辐射试验, 分析007^T 对紫外线和γ射线辐射的抗性。结果表明，AS1.633和007^T的生长特性和形态特征有所不同，AS1.633为橙红色光滑、边缘整齐的菌落，而007^T为鲜红色菌落，边缘不整齐，菌落表面干燥、呈褶皱。耐辐射试验表明007^T 对紫外线辐射存活最高剂量是624Jｍ^-2，存活率为4%； γ射线16kGy照射后，007^T存活率为6%，说明007^T具有的极强的辐射抗性。

Abstract:

Studies were carried out to characterize and compare the morphology, cultivation and radiation resistance of 007^T to type strain AS1.633 as well as the known radiation resistant organism Deinococcus radiodurans R1. To determine the levels of radio resistance, culture of bacteria 007^T by comparing with cultures of bacteria AS1.633 and E. coli DH5α as well as the known radiation resistant and radiation sensitive organisms Deinococcus radiodurans R1 and Escherichia coli, respectively, were irradiated at the desired doses of UV and ⁶⁰Co gamma radiation. Results showed some difference of the morphology and cultivation characteristics of AS1.633 and 007^T. The colonies of AS1.633 were orange-red and smooth, whereas the colonies of 007^T were bright red and usually dry with pleats. Strain 007^T showed strongly resistant to UV and ⁶⁰Co gamma radiation compared to the radiation resistant organism D. radiodurans R1 and the radiation sensitive organism E.coli DH5α, 4% survival after exposition to UV-doses as high as 624Jm^-2 and 6% survival after radiated by 16kGy,respectively.