双歧杆菌肽聚糖结构及分子量的分析

Structure and Molecular Weight Analysis of Bifidobacterium Peptidoglycan

摘要:

从双歧杆菌细胞壁中分离纯化肽聚糖，研究其化学成分、结构和分子量分析。经氨基酸、多糖和蛋白质含量分析和肽聚糖的溶解实验鉴定，所提取物质的主要成分为肽聚糖；经核磁共振和红外光谱分析，所提取肽聚糖的结构与已知的肽聚糖的结构相吻合，其糖链结构为由D型吡喃糖N-乙酰葡糖胺和N-乙酰胞壁酸以β-1,4-糖苷键连接而组成；经SDS-PAGE和多种染色方法结合，分析经溶菌酶水解后的肽聚糖，其分子量的分布呈弥散状，范围在97.6 kD到14.4 kD之间。

Abstract:

PG derived from Bifidobacterium thermophilum was separated and purified from the cell wall, and involved in research and analysis of its chemical compositions, structure and molecular weight. The major component of the preparation was validated to be peptidoglycan by animo acid analysis, polysaccharides and protein determination, dissolution by lysozyme. NMR and Infrared spectrum substantiated the glycan chain of peptidoglycan was connected by D-pyranose N-acetylglucosamine and N-acetylmuramic acid through β-1,4 glycosidic bond. SDS-PAGE and multi-stain suggested that the molecular weight of soluble peptidoglycan showed certain diffusibility, and its ranged from lower than 97.6 kD to higher than 14.4 kD.